Flanagan Kelly E, Tien Lee W, Elia Roberto, Wu Julian, Kaplan David
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts.
J Biomed Mater Res B Appl Biomater. 2015 Apr;103(3):485-94. doi: 10.1002/jbm.b.33217. Epub 2014 Jun 12.
Silk solvent casting, electrospinning, and electrogelation techniques were used to create a biodegradable, biocompatible silk fibroin dural substitute. The all-silk system was designed and produced to improve on currently available materials, grafts and tissue sealants used for dural closure in neurosurgery. The silk biomaterial was successfully fabricated as a dual layer adhesive system designed to seal durotomies while also functioning as a dural regeneration scaffold. The mechanical characteristics, biocompatibility, biodegradability, and hydrodynamic sealing capability of the material were evaluated. Results showed that the biomaterial was biocompatible with neural cells and fibroblasts, had mechanical properties mimicking the natural dura, was biodegradable with controllable degradation, and was able to seal against a hydrodynamic pressure of 205 mmHg, which greatly exceeds the maximum cerebrospinal fluid pressure seen in both cranial and spinal dural closures of 50 mmHg. Based on its design and experimental results, the adhesive silk dual layer composite biomaterial shows potential as a sutureless dural repair system that would improve on current dural closure techniques.
采用丝素溶剂浇铸、静电纺丝和电凝胶化技术制备了一种可生物降解、生物相容的丝素蛋白硬脑膜替代物。设计并生产了全丝系统，以改进目前神经外科手术中用于硬脑膜闭合的材料、移植物和组织密封剂。成功制备了一种丝素生物材料双层粘合剂系统，用于封闭硬脑膜切开术，同时还作为硬脑膜再生支架发挥作用。对该材料的力学特性、生物相容性、生物降解性和流体动力密封能力进行了评估。结果表明，该生物材料与神经细胞和成纤维细胞具有生物相容性，具有模拟天然硬脑膜的力学性能，可生物降解且降解可控，能够承受205 mmHg的流体动力压力，这大大超过了颅骨和脊柱硬脑膜闭合中所见的最大脑脊液压力50 mmHg。基于其设计和实验结果，这种粘性丝素双层复合生物材料显示出作为一种无缝合硬脑膜修复系统的潜力，有望改进当前的硬脑膜闭合技术。
