Department of Medicine, Section of Pulmonary and Critical Care, Department of Anesthesia and Critical Care and
Department of Medicine, Section of Pulmonary and Critical Care.
Br J Anaesth. 2014 Jul;113 Suppl 1(Suppl 1):i103-8. doi: 10.1093/bja/aeu165. Epub 2014 Jun 11.
We and others have previously demonstrated that the μ-opioid receptor (MOR) is overexpressed in several human malignancies. There is a seven-fold increase in MOR in cell lines of human lung cancer. In animal models, overexpression of MOR promotes tumour growth and metastasis. We, therefore, examined whether MOR expression is increased in metastatic lung cancer.
In this study, we examined the association between MOR expression and metastasis in archived biopsy samples from patients with lung cancer. Paraffin-embedded patient material was stained using MOR antibody and scored qualitatively by two independent pathologists using a four-point scale.
In human lung cancer and normal adjacent lung samples obtained from 34 lung cancer patients, MOR expression was increased significantly in cancer samples from patients with lung cancer compared with adjacent control tissue (P=0.0242). When the samples from patients with metastatic lung cancer were separated from the cohort of the total number of patients with lung cancer, we observed an approximately two-fold increase in MOR expression (P=0.0013).
The association between the expression of MOR and the progression of the tumour is consistent with the hypothesis of a direct effect of MOR on cancer progression.
我们和其他人之前已经证明 μ-阿片受体（MOR）在几种人类恶性肿瘤中过度表达。在人类肺癌细胞系中，MOR 的表达增加了七倍。在动物模型中，MOR 的过度表达促进肿瘤生长和转移。因此，我们研究了转移性肺癌中 MOR 表达是否增加。
在这项研究中，我们检查了 MOR 表达与存档的肺癌患者活检样本中转移之间的关联。使用 MOR 抗体对石蜡包埋的患者标本进行染色，并由两位独立的病理学家使用四点量表进行定性评分。
在从 34 名肺癌患者获得的人肺癌和正常相邻肺样本中，与相邻对照组织相比，来自肺癌患者的癌症样本中 MOR 表达显著增加（P=0.0242）。当将来自转移性肺癌患者的样本与肺癌患者总数的队列分开时，我们观察到 MOR 表达增加了大约两倍（P=0.0013）。
MOR 表达与肿瘤进展之间的关联与 MOR 对癌症进展的直接影响的假设一致。
