Senol F Sezer, Acikara Ozlem Bahadir, Citoglu Gulcin Saltan, Orhan Ilkay Erdogan, Dall' Acqua Stefano, Ozgökce Fevzi
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University , Ankara , Turkey .
Pharm Biol. 2014 Jul;52(7):873-82. doi: 10.3109/13880209.2013.872152. Epub 2014 Feb 20.
Scorzonera L. species (Asteraceae) are edible and as medicinal plants are used for various purposed in folk medicine.
The methanol extracts of the aerial parts and roots from 27 Scorzonera taxa were investigated for their possible neurobiological effects.
Inhibitory potential of the Scorzonera species was tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYRO) at 100 µg mL(-1) using ELISA microtiter assay. Antioxidant activity of the extracts was tested with radical scavenging activity, metal-chelation capacity, ferric- (FRAP), and phosphomolibdenum-reducing antioxidant power (PRAP) assays. Chlorogenic acid, hyperoside, rutin, and scorzotomentosin-4-O-β-glucoside were also screened in the same manner. Total phenol and flavonoid quantification in the extracts were determined spectrophotometrically.
The aerial parts of Scorzonera pisidica (40.25 ± 0.74%) and chlorogenic acid (46.97 ± 0.82%) displayed the highest TYRO inhibition, while the remaining samples showed only trivial inhibition against cholinesterases (2.08 ± 1.35%-25.32 ± 1.37%). The same extract of S. pisidica was revealed to be the most potent in scavenging of all three radicals and FRAP assay.
Out of 27 taxa, S. pisidica, in particular, may deserve further investigation for its neuroprotective potential.
鸦葱属植物（菊科）可食用，作为药用植物在民间医学中有多种用途。
研究27种鸦葱类群地上部分和根部的甲醇提取物可能的神经生物学效应。
采用ELISA微量滴定法，在100μg/mL浓度下测试鸦葱属植物对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和酪氨酸酶（TYRO）的抑制潜力。通过自由基清除活性、金属螯合能力、铁还原抗氧化能力（FRAP）和磷钼酸还原抗氧化能力（PRAP）测定来测试提取物的抗氧化活性。绿原酸、金丝桃苷、芦丁和鸦葱素-4-O-β-葡萄糖苷也以同样的方式进行筛选。用分光光度法测定提取物中的总酚和黄酮含量。
皮西迪亚鸦葱地上部分（40.25±0.74%）和绿原酸（46.97±0.82%）对酪氨酸酶的抑制作用最强，而其余样品对胆碱酯酶的抑制作用较弱（2.08±1.35%-25.32±1.37%）。皮西迪亚鸦葱的同一提取物在清除所有三种自由基和FRAP测定中显示出最强的活性。
在27个类群中，皮西迪亚鸦葱因其神经保护潜力尤其值得进一步研究。
