Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
Food Chem. 2013 Nov 1;141(1):582-8. doi: 10.1016/j.foodchem.2013.03.020. Epub 2013 Mar 13.
In vitro neuroprotective activity of the extracts of Viburnum tinus L. was investigated via inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and tyrosinase (TYRO) by microtitre plate assays. Their antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), super oxide (SO), and nitric oxide (NO) radical-scavenging activities, ferric ion-chelation capacity, ferric- (FRAP), and phosphomolybdenum-reducing antioxidant power (PRAP) assays. Total phenol and flavonoid content of the extracts was determined spectrophotometrically. The branch-ethyl acetate and fruit-methanol extracts exerted potent anticholinesterase effects (66.4 ± 0.65% to 97.7 ± 0.47%), while the fruit-methanol extract had the highest TYRO inhibition (47.0 ± 0.68%). The methanol extracts showed higher activities in most of the antioxidant tests. All the extracts displayed notable NO-scavenging effects (47.5 ± 5.03% to 74.5 ± 1.80%). Only the fruit-ethyl acetate extract quenched SO radical (38.4 ± 1.01%) at 500 μg ml(-1). Our data indicate that the fruit and branch extracts of V. tinus may provide potential neuroprotection.
采用微量板分析方法，研究了丁香叶悬钩子提取物对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）以及酪氨酸酶（TYRO）的抑制作用，来研究其体外神经保护活性。通过 2,2-二苯基-1-苦肼基（DPPH）、N,N-二甲基-对苯二胺（DMPD）、超氧化物（SO）和一氧化氮（NO）自由基清除活性、铁离子螯合能力、铁（FRAP）和磷酸钼还原抗氧化能力（PRAP）测定法来测试其抗氧化活性。采用分光光度法测定提取物中总酚和类黄酮的含量。枝乙酸乙酯和果实甲醇提取物表现出很强的抗胆碱酯酶作用（66.4±0.65%至 97.7±0.47%），而果实甲醇提取物对 TYRO 的抑制作用最高（47.0±0.68%）。甲醇提取物在大多数抗氧化试验中表现出更高的活性。所有提取物对 NO 清除均有显著作用（47.5±5.03%至 74.5±1.80%）。只有果实乙酸乙酯提取物能在 500μg ml(-1)时淬灭 SO 自由基（38.4±1.01%）。我们的数据表明，丁香叶悬钩子的果实和树枝提取物可能提供潜在的神经保护作用。
