Nanetti Laura, Raffaelli Francesca, Giulietti Alessia, Sforza Giulia, Raffaele Giannubilo Stefano, Ciavattini Andrea, Tranquilli Andrea Luigi, Mazzanti Laura, Vignini Arianna
Section of Biochemistry, Biology and Physics and.
J Matern Fetal Neonatal Med. 2015 Mar;28(5):611-6. doi: 10.3109/14767058.2014.927859. Epub 2014 Jul 11.
The aim of the present study was to understand the role played by Atosiban, an oxytocin receptor antagonist, on trophoblastic human cells, and the molecular bases of its efficacy and safety in the treatment of preterm labor. NO, peroxinitrite production and NOS expression have been evaluated on placenta obtained from term and preterm labors.
We studied trophoblast cells isolated from selected placental tissue from 20 controls and 20 preterm patients after cesarean sections. Each sample was studied at basal state and after 2 hours incubation with oxytocin and Atosiban.
Significant variations of NO levels, peroxynitrite production and iNOS and eNOS expression both in the preterm, term samples and in each of the considered groups were observed. In the control group Atosiban re-established NO levels that were reduced after incubation with oxytocin, while in preterm samples NO levels were not only re-established but, after incubation with Atosiban, significantly increased compared to basal levels.
This confirms the beneficial role of Atosiban in prolonging the pregnancy of spontaneous labor at very early gestational periods. In conclusion, Atosiban might be an effective drug to prevent preterm labor, in the therapeutic approach to this pathology.
本研究旨在了解催产素受体拮抗剂阿托西班对人滋养层细胞的作用，以及其在治疗早产中疗效和安全性的分子基础。对足月产和早产胎盘的一氧化氮（NO）、过氧亚硝酸盐生成及一氧化氮合酶（NOS）表达进行了评估。
我们研究了从20例剖宫产术后对照产妇和20例早产产妇的选定胎盘组织中分离出的滋养层细胞。每个样本在基础状态下以及与催产素和阿托西班孵育2小时后进行研究。
在早产样本、足月样本以及每个研究组中均观察到NO水平、过氧亚硝酸盐生成以及诱导型NOS（iNOS）和内皮型NOS（eNOS）表达的显著变化。在对照组中，阿托西班使与催产素孵育后降低的NO水平恢复正常，而在早产样本中，NO水平不仅恢复正常，而且与基础水平相比，在与阿托西班孵育后显著升高。
这证实了阿托西班在极早期妊娠中延长自然分娩孕周方面的有益作用。总之，在早产的治疗中，阿托西班可能是预防早产的一种有效药物。
