Zheng Danwei, Liao Shan, Zhu Guangchao, Zhou Yanhong, Li Guiyuan
Institute of Cancer Research, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 May;39(5):525-31. doi: 10.3969/j.issn.1672-7347.2014.05.014.
In recent years, cancer stem cells have become a hotspot for global researchers. Cancer stem cell theory deems that cells with self-renewal and differentiation potential play a key role in tumor resistance and relapse. These cells are named cancer stem cells. At present, the sorting methods include the side population cell sorting technique, screening techniques based on cell surface special markers, tumor sphere cultures, label retaining cell, ALDEFLUOR assays and so on. Side population cells are a small part of cells with the capacity of efflux DNA fluorescent dye Hoechst 33342 and present a low staining intensity in flow cytometry plot. Side population cells are rich in cancer stem cells, and its sorting method has been considered simple and effective in cancer stem cell research.
近年来，癌症干细胞已成为全球研究人员的热点。癌症干细胞理论认为，具有自我更新和分化潜能的细胞在肿瘤耐药性和复发中起关键作用。这些细胞被称为癌症干细胞。目前，分选方法包括侧群细胞分选技术、基于细胞表面特殊标志物的筛选技术、肿瘤球培养、标记保留细胞、ALDEFLUOR检测等。侧群细胞是一小部分具有外排DNA荧光染料Hoechst 33342能力的细胞，在流式细胞术图中呈现低染色强度。侧群细胞富含癌症干细胞，其分选方法在癌症干细胞研究中被认为简单有效。
