霍乱弧菌O1群CT基因阴性菌株产生的新型霍乱毒素之间的免疫生物学关系。

Immunobiological relationships among new cholera toxins produced by CT gene-negative strains of Vibrio cholerae O1.

作者信息

Saha S, Sanyal S C

机构信息

Department of Microbiology, Banaras Hindu University, Varanasi, India.

出版信息

J Med Microbiol. 1989 Jan;28(1):33-7. doi: 10.1099/00222615-28-1-33.

DOI:10.1099/00222615-28-1-33

PMID:2492345

Abstract

The enterotoxic activities of partially purified new cholera toxins (NCT) prepared from the CT gene-negative strains of Vibrio cholerae serotype O1 isolated from cases of diarrhoea in man and from diverse environmental sources were assayed in the rabbit ileal-loop model and the toxin unit was calculated in micrograms of protein content. The secretory activities of one unit of homologous and heterologous enterotoxins were completely neutralised by 2.5 units of crude antiserum raised against one NCT preparation. In immunodiffusion tests, the NCT preparations from all strains tested gave precipitin bands against one antiserum showing reaction of complete identity. Thus, the present study clearly demonstrated that NCTs elaborated by CT gene-negative strains of V. cholerae O1 are immunobiologically similar.

摘要

对从人类腹泻病例及不同环境来源分离出的霍乱弧菌O1血清型CT基因阴性菌株制备的部分纯化新型霍乱毒素（NCT）的肠毒素活性，采用兔回肠袢模型进行测定，并以蛋白质含量的微克数计算毒素单位。针对一种NCT制剂制备的2.5单位粗抗血清可完全中和一个单位同源和异源肠毒素的分泌活性。在免疫扩散试验中，所有测试菌株的NCT制剂与一种抗血清均产生沉淀带，显示完全相同的反应。因此，本研究清楚地表明，霍乱弧菌O1的CT基因阴性菌株产生的NCT在免疫生物学上是相似的。