Saha S, Sanyal S C
Indian J Med Res. 1989 May;89:117-20.
The enterotoxicity of the new cholera toxin (NCT) prepared from cholera toxin gene-negative (CT-) V. cholerae 01 strains isolated from human diarrhoeal and environmental sources was assayed in rabbit ileal loops and the toxin unit was calculated to be 24 micrograms of protein. The enterotoxicity of the NCT preparations were completely neutralised by the antiserum raised against the enterotoxin preparation from the CT+ V. cholerae 01 strain 569B at 1 in 16 dilution in ileal loops. The antiserum contained 1 unit of antitoxin in 85 x 10(-4) ml amount. The data indicate that the antiserum prepared against the enterotoxin of CT+ strain contains antibody against the NCT and can neutralise the toxin in vivo. The observations also suggest that CT+ strains liberate the NCT simultaneously with CT and the latter gets eliminated during the process of purification.
从人类腹泻和环境来源分离的霍乱毒素基因阴性（CT-）霍乱弧菌01菌株制备的新型霍乱毒素（NCT）的肠毒性，在兔回肠袢中进行了测定，毒素单位经计算为24微克蛋白质。在回肠袢中，NCT制剂的肠毒性被针对CT+霍乱弧菌01菌株569B的肠毒素制剂产生的抗血清以1:16稀释完全中和。该抗血清在85×10（-4）毫升量中含有1单位抗毒素。数据表明，针对CT+菌株肠毒素制备的抗血清含有针对NCT的抗体，并且可以在体内中和毒素。这些观察结果还表明，CT+菌株在释放霍乱毒素（CT）的同时释放NCT，而后者在纯化过程中被去除。
