Hiremath Hemalatha, Kulkarni Sadanand, Sharma Robin, Hiremath Vishwanath, Motiwala Tejas
Department of Conservative Dentistry and Endodontics, SAIMS, Indore, India.
Dent Traumatol. 2014 Dec;30(6):442-6. doi: 10.1111/edt.12119. Epub 2014 Jun 13.
The prognosis of replanted avulsed tooth depends on the existence of viable cells in the periodontal ligament and also on those cells which are able to proliferate on the damaged areas of the root. The purpose of this study was to evaluate the survival of periodontal ligament cells (PDL) when soaked in an autologous biologic rejuvenating media after an extra-oral dry time of 40 min.
Thirty teeth were selected with intact crown which were advised for Orthodontic extraction having healthy PDL. They were divided into two experimental and two control groups. The positive and negative controls corresponded to 0-min and 1-h dry time, respectively. The experimental teeth were stored dry for 40 min and then immersed in one of the two media, combination of platelet-rich fibrin and platelet poor plasma (PRF+PPP) and PPP for 45 min. The teeth in each group were treated with dispase II and collagenase for 30 min and later centrifuged for 5 min at 50.17 g. The supernatant was removed with sterile micropipette, the cells labelled with 0.4% trypan blue, and the number of viable PDL cells was counted with a haemocytometer, under a light microscope.
anova and Mann-Whitney U-test demonstrated statistically significant differences in the viability of PDL cells among experimental groups.
Within the parameters of this study, a combination of platelet-rich fibrin and PPP demonstrated higher number of viable PDL cells and hence could be a good biologic rejuvenating media for avulsed teeth.
再植撕脱牙的预后取决于牙周膜中活细胞的存在，也取决于那些能够在牙根受损区域增殖的细胞。本研究的目的是评估在口腔外干燥40分钟后，牙周膜细胞（PDL）浸泡于自体生物复壮培养基中的存活率。
选择30颗冠完整、建议正畸拔除且牙周膜健康的牙齿。将它们分为两个实验组和两个对照组。阳性和阴性对照分别对应0分钟和1小时的干燥时间。实验牙齿干燥保存40分钟，然后浸入两种培养基之一，即富血小板纤维蛋白和贫血小板血浆的组合（PRF+PPP）和PPP中45分钟。每组牙齿用Ⅱ型胶原酶和胶原酶处理30分钟，然后以50.17g离心5分钟。用无菌微量移液器吸出上清液，用0.4%台盼蓝标记细胞，并在光学显微镜下用血细胞计数器计数存活的PDL细胞数量。
方差分析和曼-惠特尼U检验表明，实验组之间PDL细胞活力存在统计学显著差异。
在本研究的参数范围内，富血小板纤维蛋白和PPP的组合显示出更多存活的PDL细胞，因此可能是一种用于撕脱牙的良好生物复壮培养基。
