Shetty Ashwija, Ghosh Somnath, Srirekha A, Jaykumar T, Chikkamallaiah Champa, Adiga Savitha
Department of Conservative Dentistry and Endodontics, The Oxford Dental College Hospital, Bommanhalli, India.
Department of Conservative Dentistry and Endodontics (S.G. C.C., S.Adiga), Bommanhalli, India.
Eur Endod J. 2019 Oct 21;4(3):118-121. doi: 10.14744/eej.2019.24633. eCollection 2019.
To compare the efficacy of platelet-rich fibrin (PRF) and Hank's balanced salt solution (HBSS) in the preservation of the periodontal ligament (PDL) cells viability of avulsed teeth.
A total of 30 non-carious third molars with healthy periodontium, indicated for extraction for orthodontic reasons or chronic pericoronitis, were selected for the study. Samples were divided into four groups: one standard group and one experimental group in addition to two control groups (positive and negative). The positive and negative control group corresponded to immediate and 2-hour dry time respectively. The experimental teeth were bench dried for 40 minutes and then immersed in one of the two storage media: HBSS (standard storage media) and PRF (experimental storage media) for 45 minutes. The teeth in each group were treated with dispase II and collagenase for 30 minutes and later centrifuged for 4 minutes at 1000 rpm. The supernatant was removed with sterile micropipette, the cells were labeled with 0.4% trypan blue, and the number of viable PDL cells was counted with a hemocytometer under a light microscope. One-way Kruskal-Wallis test and Mann-Whitney U test with Boneferroni correction were used for statistical analysis.
Results did not demonstrate any statistically significant differences in the viability of PDL cells between the groups with standard and experimental storage media. Group 1 showed a statistically significant difference of mean compared to Groups 2, 3 and 4. When Group 2 was compared with Groups 3 and 4, a P-value>0.05 suggested no statistical significance.
Within the parameters of this study, HBSS and PRF demonstrated a similar number of viable PDL cells. Hence, PRF could be a used as a good substitute of HBSS as a storage media for avulsed teeth.
比较富血小板纤维蛋白（PRF）和汉克平衡盐溶液（HBSS）在保存脱位牙牙周膜（PDL）细胞活力方面的效果。
选取30颗因正畸原因或慢性冠周炎需拔除的、牙周组织健康的非龋第三磨牙用于本研究。样本分为四组：除两个对照组（阳性和阴性）外，还有一个标准组和一个实验组。阳性和阴性对照组分别对应立即处理和干燥2小时。将实验牙在实验台上干燥40分钟，然后浸入两种储存介质之一：HBSS（标准储存介质）和PRF（实验储存介质）中45分钟。每组牙齿用Ⅱ型胶原酶和胶原酶处理30分钟，随后以1000转/分钟的速度离心4分钟。用无菌微量移液器吸出上清液，细胞用0.4%台盼蓝染色，在光学显微镜下用血细胞计数器计数存活的PDL细胞数量。采用单向Kruskal-Wallis检验和经Bonferroni校正的Mann-Whitney U检验进行统计分析。
结果显示，标准储存介质组和实验储存介质组之间的PDL细胞活力无统计学显著差异。第1组与第2、3和4组相比，平均差异具有统计学意义。当第2组与第3和4组比较时，P值>0.05表明无统计学意义。
在本研究的参数范围内，HBSS和PRF显示出相似数量的存活PDL细胞。因此，PRF可作为HBSS的良好替代品，用作脱位牙的储存介质。
