Weber F L, Fresard K M, Veach G L
Veterans Administration Medical Center, Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
Gastroenterology. 1989 Mar;96(3):935-7.
This study was conducted to determine whether short-term (1.75 h) luminal glucose perfusion increases the mucosal protein synthesis rate in rat small intestine. A luminal perfusate containing 56 mM glucose was compared with a control perfusate containing mannitol in two jejunal segments constructed in the same animal. Mucosal protein synthesis rates were determined when the tracer amino acid was administered intravenously and intraluminally. The results indicated that luminal glucose perfusion rapidly stimulated mucosal protein synthesis in the fed state by 20% and 37% with the labeled amino acid derived from the vascular and luminal compartment, respectively. A 16-h fast abolished the stimulatory effect of glucose when the labeled amino acid was given intravascularly but not intraluminally. These effects of glucose could be ascribed to a direct alteration of mucosal metabolism rather than to indirect systemic effects.
本研究旨在确定短期（1.75小时）肠腔葡萄糖灌注是否会增加大鼠小肠黏膜蛋白合成率。在同一动物构建的两段空肠中，将含56 mM葡萄糖的肠腔灌注液与含甘露醇的对照灌注液进行比较。当通过静脉内和肠腔内给予示踪氨基酸时，测定黏膜蛋白合成率。结果表明，在进食状态下，肠腔葡萄糖灌注分别使来自血管和肠腔的标记氨基酸的黏膜蛋白合成迅速增加20%和37%。当血管内给予标记氨基酸而非肠腔内给予时，16小时禁食消除了葡萄糖的刺激作用。葡萄糖的这些作用可归因于黏膜代谢的直接改变，而非间接的全身作用。
