Chang M, Hadero A, Crawford I P
Department of Microbiology, University of Iowa, Iowa City 52242.
J Bacteriol. 1989 Jan;171(1):172-83. doi: 10.1128/jb.171.1.172-183.1989.
In Pseudomonas aeruginosa, the trpI gene product regulates the expression of the trpBA gene pair encoding tryptophan synthase. trpI and trpBA are transcribed divergently. The trpI DNA sequence and deduced amino acid sequence were determined. The trpI start codon was found to be 103 base pairs from that of trpB. trpI encodes a 293-residue protein and the size of the trpI gene product, measured on sodium dodecyl sulfatepolyacrylamide gels, was close to that calculated from the amino acid sequence. The amino acid sequence of trpI resembles that of Enterobacter cloacae ampR, the regulatory gene for the ampC cephalosporinase. The N-terminal portions of trpI and ampR resemble corresponding portions of ilvY, metR, and lysR in Escherichia coli and nodD in Rhizobium meliloti. This resemblance may help to define a trpI-related family of activator proteins sharing a common structural plan.
在铜绿假单胞菌中，trpI基因产物调节编码色氨酸合酶的trpBA基因对的表达。trpI和trpBA以相反方向转录。测定了trpI的DNA序列和推导的氨基酸序列。发现trpI起始密码子与trpB的起始密码子相距103个碱基对。trpI编码一个293个残基的蛋白质，在十二烷基硫酸钠-聚丙烯酰胺凝胶上测定的trpI基因产物大小与根据氨基酸序列计算出的大小相近。trpI的氨基酸序列类似于阴沟肠杆菌ampR（ampC头孢菌素酶的调节基因）的氨基酸序列。trpI和ampR的N端部分类似于大肠杆菌中ilvY、metR和lysR以及苜蓿根瘤菌中nodD的相应部分。这种相似性可能有助于定义一个具有共同结构模式的与trpI相关的激活蛋白家族。
