Department of Dermatology, Chonnam National University Medical School and Hospital, Gwangju, Korea.
Department of Chemical Engineering, Sunchon National University, Chonnam, Korea.
Invest Ophthalmol Vis Sci. 2014 Jun 12;55(7):4119-27. doi: 10.1167/iovs.13-13441.
To investigate the effects of visible light on human corneal epithelial cells and the impact of natural antioxidants on oxidative stress produced by overexposure to light.
Light-emitting diodes with various wavelengths (410-830 nm) were used to irradiate human corneal epithelial cells, and cell viability was assessed. The production of reactive oxygen species (ROS) was analyzed using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Ethyl alcohol (EtOH) extracts were prepared from mixtures of medicinal plants. After application of the EtOH extracts, the free radical scavenging activity was measured using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The induction of antioxidant enzymes including heme oxygenase-1 (HO-1), peroxiredoxin-1 (Prx-1), catalase (CAT), and superoxide dismutase-2 (SOD-2) by the extracts was evaluated by reverse transcription-polymerase chain reaction and Western blotting. The ability of the extracts to inhibit ROS was also analyzed using DCF-DA.
The viability of corneal epithelial cells was diminished after irradiation of blue light (above 10 J at 410 nm and 50 J at 480 nm). Reactive oxygen species production was induced by irradiation at 410 and 480 nm at doses of 5 J/cm(2) and higher. Ethyl alcohol extracts had potent radical scavenging activity. Application of the extracts not only increased the expression of HO-1, Prx-1, CAT, and SOD-2, but it also attenuated the ROS production induced by blue light in a dose-dependent manner.
Overexposure to blue light (410-480 nm) may have a harmful effect on human corneal epithelial cells compared with other visible light wavelengths. Medicinal plant extracts can have potent protective effects on blue light-induced oxidative stress.
研究可见光对人眼角膜上皮细胞的影响，以及天然抗氧化剂对光过度暴露产生的氧化应激的影响。
使用不同波长（410-830nm）的发光二极管照射人眼角膜上皮细胞，评估细胞活力。使用 2'，7'-二氯二氢荧光素二乙酸酯（DCF-DA）分析活性氧（ROS）的产生。从药用植物混合物中制备乙醇（EtOH）提取物。应用 EtOH 提取物后，使用 2,2-二苯基-1-苦基肼（DPPH）自由基清除测定法测量自由基清除活性。通过逆转录-聚合酶链反应和 Western 印迹评估提取物对血红素加氧酶-1（HO-1）、过氧化物酶-1（Prx-1）、过氧化氢酶（CAT）和超氧化物歧化酶-2（SOD-2）等抗氧化酶的诱导作用。还使用 DCF-DA 分析了提取物抑制 ROS 的能力。
蓝光（410nm 时超过 10J，480nm 时超过 50J）照射后，角膜上皮细胞活力降低。在 410nm 和 480nm 下，剂量为 5J/cm2 及更高时，会诱导 ROS 产生。乙醇提取物具有很强的自由基清除活性。提取物的应用不仅增加了 HO-1、Prx-1、CAT 和 SOD-2 的表达，而且还以剂量依赖的方式减弱了蓝光诱导的 ROS 产生。
与其他可见光波长相比，过度暴露于蓝光（410-480nm）可能对人眼角膜上皮细胞有害。药用植物提取物对蓝光诱导的氧化应激具有很强的保护作用。
