Lee Hyo Seok, Choi Joo-Hee, Cui Lian, Li Ying, Yang Jee Myung, Yun Je-Jung, Jung Ji Eun, Choi Won, Yoon Kyung Chul
Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, South Korea.
College of Veterinary Medicine, BK 21 PLUS Project Team, Chonnam National University, Gwangju, South Korea.
Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):1196-1207. doi: 10.1167/iovs.16-20634.
To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE).
Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganese-dependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis. Furthermore, eye drops containing 0.001%, 0.01%, or 0.1% CJ extract or a balanced salt solution (BSS) were applied to the EDE. Clinical parameters were measured 7 days after treatment. The levels of inflammatory markers and intracellular reactive oxygen species (ROS) were measured.
Treatment with 0.01% and 0.1% CJ extracts decreased apoptosis in HCE cells. In addition, band intensities of PRX 1, 4, and 5, as well as MnSOD, after hydrogen peroxide (H2O2) treatment showed a significant improvement after pretreatment with 0.01% and 0.1% CJ extracts. Mice treated with 0.1% CJ extract showed significantly improved clinical parameters when compared to those of the EDE control and BSS groups. A significant decrease in the levels of inflammatory markers and intracellular ROS was observed in the 0.01% and 0.1% CJ extract groups.
Camellia japonica extracts promoted antioxidative protein expression and suppressed apoptosis in HCE cells. Furthermore, CJ extracts improved clinical signs of dry eye and reduced oxidative stress and the expression of inflammatory markers, suggesting that eye drops containing CJ extract could be used as an adjunctive treatment for dry eye.
分析山茶花（CJ）对人角膜上皮（HCE）细胞的抗炎和抗氧化作用及其在实验性干眼症（EDE）小鼠模型中的治疗效果。
用不同浓度（0.001%、0.01%和0.1%）的山茶花提取物处理HCE细胞。进行二氯荧光素二乙酸酯（DCF-DA）和二氢乙锭（DHE）检测。采用蛋白质免疫印迹分析评估HCE细胞中过氧化物还原酶（PRX）1-6和锰依赖性超氧化物歧化酶（MnSOD）的产生。此外，将含有0.001%、0.01%或0.1%CJ提取物或平衡盐溶液（BSS）的滴眼液应用于EDE模型。治疗7天后测量临床参数。测量炎症标志物和细胞内活性氧（ROS）水平。
用0.01%和0.1%CJ提取物处理可减少HCE细胞凋亡。此外，用过氧化氢（H2O2）处理后，0.01%和0.1%CJ提取物预处理后PRX 1、4和5以及MnSOD的条带强度显著改善。与EDE对照组和BSS组相比，用0.1%CJ提取物处理的小鼠临床参数显著改善。在0.01%和0.1%CJ提取物组中观察到炎症标志物和细胞内ROS水平显著降低。
山茶花提取物促进抗氧化蛋白表达并抑制HCE细胞凋亡。此外，CJ提取物改善了干眼症的临床症状，降低了氧化应激和炎症标志物的表达，表明含有CJ提取物的滴眼液可作为干眼症的辅助治疗药物。
