Zhang Xiaoli, Wu Yin, Sun Kun, Tan Jing
Department of Rheumatism and Nephropathy, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
Exp Ther Med. 2014 Jun;7(6):1659-1662. doi: 10.3892/etm.2014.1643. Epub 2014 Mar 28.
The aim of the present study was to investigate the effect of erythropoietin (EPO) loading chitosan-tripolyphosphate (CS-TPP) nanoparticles on an immunoglobulin A nephropathy (IgAN) rat model. CS-TPP nanoparticles were produced from CS and TPP and EPO was loaded by mixing with the nanoparticles. The IgAN rat models were randomly divided into three groups: the CS-TPP-EPO group, CS-TPP group and EPO group. Hemoglobin (Hb), blood urea nitrogen (BUN) and creatinine (Cr) levels were measured in each group using a Biochemical Analyzer (Hitachi, Tokyo, Japan). The average size of nanoparticles was 485±12 nm and the encapsulation efficiency of EPO was 78.45%. The EPO release curve in CS-TPP-EPO nanoparticles exhibited a biphasic distribution . The levels of BUN and Cr in the CS-TPP-EPO group were significantly lower compared with the control group (P<0.05); however, the level of Hb in the CS-TPP-EPO group was higher compared with the other groups (P<0.05). The changes in Hb, BUN and Cr in the CS-TPP-EPO group were maintained for less than one week following the end of the treatment with CS-TPP-EPO nanoparticles. In conclusion, the CS-TPP-EPO nanoparticles had a lower toxicity compared with EPO and CS-TPP treatment. Furthermore, CS-TPP-EPO may improve the therapeutic effect in the IgAN model. This suggests that CS-TPP-EPO nanoparticles may be a potential therapeutic drug for the treatment of patients with IgAN.
本研究的目的是探讨负载促红细胞生成素（EPO）的壳聚糖-三聚磷酸钠（CS-TPP）纳米颗粒对免疫球蛋白A肾病（IgAN）大鼠模型的影响。CS-TPP纳米颗粒由CS和TPP制备而成，通过与纳米颗粒混合负载EPO。将IgAN大鼠模型随机分为三组：CS-TPP-EPO组、CS-TPP组和EPO组。使用生化分析仪（日本东京日立公司）测量每组的血红蛋白（Hb）、血尿素氮（BUN）和肌酐（Cr）水平。纳米颗粒的平均粒径为485±12 nm，EPO的包封率为78.45%。CS-TPP-EPO纳米颗粒中的EPO释放曲线呈双相分布。与对照组相比，CS-TPP-EPO组的BUN和Cr水平显著降低（P<0.05）；然而，与其他组相比，CS-TPP-EPO组的Hb水平更高（P<0.05）。在用CS-TPP-EPO纳米颗粒治疗结束后，CS-TPP-EPO组中Hb、BUN和Cr的变化维持不到一周。总之，与EPO和CS-TPP治疗相比，CS-TPP-EPO纳米颗粒的毒性更低。此外，CS-TPP-EPO可能会提高IgAN模型的治疗效果。这表明CS-TPP-EPO纳米颗粒可能是治疗IgAN患者的一种潜在治疗药物。
