Wang Guoshan, Yu Zhigang, Zhen Yu, Mi Tiezhu, Shi Yan, Wang Jianyan, Wang Minxiao, Sun Song
College Marine Life Science, Ocean University of China, Qingdao, Shandong, P. R. China.
Key Laboratory of Marine Chemistry Theory and Engineering, Ministry of Education, Qingdao, Shandong, P. R. China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, Shandong, P. R. China.
PLoS One. 2014 Jun 13;9(6):e100057. doi: 10.1371/journal.pone.0100057. eCollection 2014.
The maintenance of physiological oxygen homeostasis is mediated by hypoxia-inducible factor (HIF), a key transcriptional factor of the PHD-HIF system in all metazoans. However, the molecular evolutionary origin of this central physiological regulatory system is not well characterized. As the earliest eumetazoans, Cnidarians can be served as an interesting model for exploring the HIF system from an evolutionary perspective. We identified the complete cDNA sequence of HIF-1α (ASHIF) from the Aurelia sp.1, and the predicted HIF-1α protein (pASHIF) was comprised of 674 amino acids originating from 2,025 bp nucleotides. A Pairwise comparison revealed that pASHIF not only possessed conserved basic helix-loop-helix (bHLH) and Per-Arnt-Sim (PAS) domains but also contained the oxygen dependent degradation (ODD) and the C-terminal transactivation domains (C-TAD), the key domains for hypoxia regulation. As indicated by sequence analysis, the ASHIF gene contains 8 exons interrupted by 7 introns. Western blot analysis indicated that pASHIF that existed in the polyps and medusa of Aurelia. sp.1 was more stable for a hypoxic response than normoxia.
生理氧稳态的维持由缺氧诱导因子（HIF）介导，HIF是所有后生动物中PHD-HIF系统的关键转录因子。然而，这个核心生理调节系统的分子进化起源尚未得到很好的表征。作为最早的真后生动物，刺胞动物可以作为从进化角度探索HIF系统的有趣模型。我们从海月水母1号（Aurelia sp.1）中鉴定出HIF-1α（ASHIF）的完整cDNA序列，预测的HIF-1α蛋白（pASHIF）由来自2025个碱基对核苷酸的674个氨基酸组成。成对比较显示，pASHIF不仅具有保守的碱性螺旋-环-螺旋（bHLH）和Per-Arnt-Sim（PAS）结构域，还包含氧依赖性降解（ODD）和C端反式激活结构域（C-TAD），这是缺氧调节的关键结构域。序列分析表明，ASHIF基因包含8个外显子，被7个内含子打断。蛋白质免疫印迹分析表明，存在于海月水母1号息肉和水母体中的pASHIF对缺氧反应比常氧更稳定。
