Ao Haiyong, Xie Youtao, Qin An, Ji Heng, Yang Shengbing, Huang Liping, Zheng Xuebin, Tang Tingting
a Shanghai Key Laboratory of Orthopedic Implant , Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine , Shanghai 200011 , China.
J Biomater Sci Polym Ed. 2014;25(11):1211-24. doi: 10.1080/09205063.2014.926577. Epub 2014 Jun 13.
In the present study, hyaluronic acid (HyA) was covalently immobilized onto titanium coatings to improve their biological properties. Diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were employed to characterize the HyA-modified titanium coating. HyA-modified titanium coatings possess better cell-material interaction, and human mesenchymal stem cells present good adhesive morphologies on the surface of TC-AAH. The results of subsequent cellular evaluation showed that the immobilization of HyA on titanium coatings could improve hMSC attachment, proliferation, and differentiation. In vivo evaluation of implants in rabbit femur condyle defect model showed improvements of early osseointegration and bone-to-implant contact of TC-AAH. In conclusion, immobilization of HyA could improve biological properties of titanium coatings.
在本研究中，将透明质酸（HyA）共价固定在钛涂层上以改善其生物学性能。采用漫反射傅里叶变换红外光谱和X射线光电子能谱对HyA修饰的钛涂层进行表征。HyA修饰的钛涂层具有更好的细胞-材料相互作用，人间充质干细胞在TC-AAH表面呈现良好的黏附形态。后续细胞评估结果表明，HyA固定在钛涂层上可改善人间充质干细胞的黏附、增殖和分化。在兔股骨髁缺损模型中对植入物进行的体内评估显示，TC-AAH的早期骨整合和骨与植入物的接触得到改善。总之，固定HyA可改善钛涂层的生物学性能。
