Rocha-Santos Vinicius, Ferro Oscar Cavalcante, Pantanali Carlos Andrés, Seixas Marcel Povlovistsch, Pecora Rafael Antonio Arruda, Pinheiro Rafael Soares, Claro Laura Carolina López, Abdo Emílio Elias, Chaib Eleazar, D'Albuquerque Luiz Augusto Carneiro
From the *Liver Transplant Unit, Department of Gastroenterology, and †Department of Pathology, Clinical Hospital of São Paulo University, School of Medicine, São Paulo, Brazil.
Pancreas. 2014 Aug;43(6):946-50. doi: 10.1097/MPA.0000000000000150.
Pancreatic ischemia-reperfusion (IR) has a key role in pancreas surgery and transplantation. Most experimental models evaluate the normothermic phase of the IR. We proposed a hypothermic model of pancreas IR to evaluate the benefic effects of the cold ischemic phase.
We performed a reproducible model of hypothermic pancreatic IR. The ischemia was induced in the pancreatic tail portion (1-hour ischemia, 4-hour reperfusion) in 36 Wistar rats. They are divided in 3 groups as follows: group 1 (control), sham; group 2, normothermic IR; and group 3, hypothermic IR. In group 3, the temperature was maintained as close to 4.5°C. After reperfusion, serum amylase and lipase levels, inflammatory mediators (tumor necrosis factor α, interleukin 6), and pancreas histology were evaluated.
In pancreatic IR groups, amylase, cytokines, and histological damage were significantly increased when compared with group 1. In the group 3, we observed a significant decrease in tumor necrosis factor α (P = 0.004) and interleukin 6 (P = 0.001) when compared with group 2. We did not observe significant difference in amylase (P = 0.867), lipase (P = 0.993), and histology (P = 0.201).
In our experimental model, we reproduced the cold phase of pancreas IR, and the pancreas hypothermia reduced the inflammatory mediators after reperfusion.
胰腺缺血再灌注（IR）在胰腺手术和移植中起关键作用。大多数实验模型评估的是IR的常温阶段。我们提出了一种胰腺IR低温模型，以评估冷缺血阶段的有益作用。
我们进行了一种可重复的胰腺低温IR模型。对36只Wistar大鼠的胰腺尾部进行缺血诱导（1小时缺血，4小时再灌注）。它们被分为以下3组：第1组（对照组），假手术组；第2组，常温IR组；第3组，低温IR组。在第3组中，温度维持在接近4.5°C。再灌注后，评估血清淀粉酶和脂肪酶水平、炎症介质（肿瘤坏死因子α、白细胞介素6）以及胰腺组织学。
与第1组相比，胰腺IR组的淀粉酶、细胞因子和组织学损伤显著增加。与第2组相比，第3组中肿瘤坏死因子α（P = 0.004）和白细胞介素6（P = 0.001）显著降低。我们未观察到淀粉酶（P = 0.867）、脂肪酶（P = 0.993）和组织学（P = 0.201）有显著差异。
在我们的实验模型中，我们重现了胰腺IR的冷相，并且胰腺低温降低了再灌注后的炎症介质。
