Food Resource Research Center, Korea Food Research Institute, Seongnam 463-746, Korea.
Research Group of Food Functionality, Korea Food Research Institute, Seongnam 463-746, Korea.
Molecules. 2014 Jun 12;19(6):7981-9. doi: 10.3390/molecules19067981.
In this study, the antidepressant-like effects of Morus alba fractions in rats were investigated in the forced swim test (FST). Male Wistar rats (9-week-old) were administered orally the M. alba ethyl acetate (EtOAc 30 and 100 mg/kg) and M. alba n-butanol fractions (n-BuOH 30 and 100 mg/kg) every day for 7 consecutive days. On day 7, 1 h after the final administration of the fractions, the rats were exposed to the FST. M. alba EtOAc fraction at the dose of 100 mg/kg induced a decrease in immobility behavior (p < 0.01) with a concomitant increase in both climbing (p < 0.05) and swimming (p < 0.05) behaviors when compared with the control group, and M. alba EtOAc fraction at the dose of 100 mg/kg decreased the hypothalamic-pituitary-adrenal (HPA) axis response to the stress, as indicated by an attenuated corticosterone response and decreased c-fos immunoreactivity in the hippocampal and hypothalamic paraventricular nucleus (PVN) region. These findings demonstrated that M. alba EtOAc fraction have beneficial effects on depressive behaviors and restore both altered c-fos expression and HPA activity.
在这项研究中，我们研究了桑白皮提取物在大鼠强迫游泳试验（FST）中的抗抑郁样作用。雄性 Wistar 大鼠（9 周龄）每天口服给予桑白皮乙酸乙酯（EtOAc 30 和 100 mg/kg）和桑白皮正丁醇（n-BuOH 30 和 100 mg/kg），连续 7 天。第 7 天，在最后一次给药后 1 小时，将大鼠暴露于 FST 中。与对照组相比，桑白皮 EtOAc 提取物 100 mg/kg 剂量可减少不动行为（p<0.01），同时增加攀爬（p<0.05）和游泳（p<0.05）行为；桑白皮 EtOAc 提取物 100 mg/kg 剂量还可减轻下丘脑-垂体-肾上腺（HPA）轴对应激的反应，表现为皮质酮反应减弱和海马及下丘脑室旁核（PVN）区域 c-fos 免疫反应性降低。这些发现表明，桑白皮 EtOAc 提取物对抑郁行为有有益的作用，并恢复了改变的 c-fos 表达和 HPA 活性。
