Department of Pharmacology, M.V.P. Samaj's College of Pharmacy, Gangapur Road, Nashik - 422002, India.
Indian J Pharmacol. 2009 Dec;41(6):246-51. doi: 10.4103/0253-7613.59921.
The objective of the present study was to evaluate the adaptogenic property of the ethyl acetate-soluble fraction of methanol extract of Morus alba roots against a rat model of chronic stress (CS).
Rats were exposed to stress procedure for 21 days. The stress procedure was mild, unpredictable footshock, administered for 1 h once daily for 21 days. Rats were administered with the ethyl acetate soluble fraction of methanol extract of M. alba roots (25, 50 and 100 mg/kg p.o) 1 h before footshock for 21 days and behavioral parameters were evaluated for cognitive dysfunction and depression using elevated plus maze and despair swim test, respectively. On day 21, rats were sacrificed immediately after stress and blood was collected for biochemical estimation. The adrenal gland and spleen were dissected for organ weight and the stomach was dissected for ulcer score.
CS significantly induced cognitive deficit, mental depression and hyperglycemia and increased blood corticosterone levels, gastric ulcerations and adrenal gland weight, but decreased the splenic weight. Pre-treatments with the ethyl acetate soluble fraction of methanol extract of M. alba roots (25, 50 and 100 mg/kg, p.o.) significantly attenuated the CS-induced perturbations. Diazepam (1 mg/kg, p.o.) was used as the standard antistress drug.
The results indicate that M. alba possesses significant adaptogenic activity, indicating its possible clinical utility as an antistress agent.
本研究旨在评估桑树根甲醇提取物乙酸乙酯可溶部分对慢性应激（CS）大鼠模型的适应原特性。
大鼠接受应激程序 21 天。应激程序为轻度、不可预测的足底电击，每天 1 次，每次 1 小时，共 21 天。大鼠在足底电击前 1 小时给予桑树根甲醇提取物乙酸乙酯可溶部分（25、50 和 100mg/kg，po），连续 21 天，并分别使用高架十字迷宫和绝望游泳试验评估认知功能障碍和抑郁的行为参数。第 21 天，大鼠在应激后立即处死，采集血液进行生化评估。解剖肾上腺和脾脏以测量器官重量，并解剖胃以测量溃疡评分。
CS 显著诱导认知障碍、精神抑郁和高血糖，并增加血液皮质酮水平、胃溃疡和肾上腺重量，但降低脾脏重量。桑树根甲醇提取物乙酸乙酯可溶部分（25、50 和 100mg/kg，po）预处理可显著减轻 CS 诱导的紊乱。地西泮（1mg/kg，po）用作标准抗应激药物。
结果表明，桑树根具有显著的适应原活性，表明其可能作为抗应激剂在临床上具有应用潜力。
