Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104-6396;
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9058-63. doi: 10.1073/pnas.1402858111. Epub 2014 Jun 9.
A constitutional isomeric library synthesized by a modular approach has been used to discover six amphiphilic Janus dendrimer primary structures, which self-assemble into uniform onion-like vesicles with predictable dimensions and number of internal bilayers. These vesicles, denoted onion-like dendrimersomes, are assembled by simple injection of a solution of Janus dendrimer in a water-miscible solvent into water or buffer. These dendrimersomes provide mimics of double-bilayer and multibilayer biological membranes with dimensions and number of bilayers predicted by the Janus compound concentration in water. The simple injection method of preparation is accessible without any special equipment, generating uniform vesicles, and thus provides a promising tool for fundamental studies as well as technological applications in nanomedicine and other fields.
通过模块化方法合成的构象异构体文库已被用于发现六种两亲性 Janus 树状大分子的初级结构，这些结构自组装成具有可预测尺寸和内部双层数量的均匀洋葱状囊泡。这些囊泡，称为洋葱状树状大分子囊泡，通过将 Janus 树状大分子在水溶性溶剂中的溶液简单注入水中或缓冲液中来组装。这些树状大分子囊泡提供了双层和多层生物膜的模拟物，其尺寸和双层数量可由水中的 Janus 化合物浓度预测。无需任何特殊设备即可采用简单的注入方法进行制备，可生成均匀的囊泡，因此为基础研究以及在纳米医学和其他领域的技术应用提供了一种很有前途的工具。
