Rojo Maria Luisa, Cipriano Mariateresa, Söderström Ingegerd, Simonytė Kotryna, Olsson Tommy, Fowler Christopher J
Department of Public Health and Clinical Medicine, Medicine, Umeå University, SE-901 87 Umeå, Sweden.
Department of Pharmacology and Clinical Neuroscience, Umeå University, SE-901 87 Umeå, Sweden.
Life Sci. 2014 Jul 17;108(2):116-21. doi: 10.1016/j.lfs.2014.05.019. Epub 2014 Jun 10.
A high consumption of fructose leads not only to peripheral changes in insulin sensitivity and vascular function, but also to central changes in several brain regions. Given the role of the endogenous cannabinoid system in the control of energy intake, we undertook a pilot study to determine whether a high fructose diet produced changes in brain CB1 receptor functionality.
Male rats given access ad libitum to normal chow were given either water, glucose or fructose solutions to drink. CB1 receptor functionality was measured autoradiographically as the increase in [(35)S]GTPγS binding produced by the agonist CP55,940.
Seven regions were investigated: the prefrontal cortex, caudate-putamen, hippocampal CA1-CA3, dentate gyrus, amygdala, and dorsomedial and ventromedial hypothalami. Two-way robust Wilcoxon analyses for each brain region indicated that the dietary treatment did not produce significant main effects upon agonist-stimulated [(35)S]GTPγS binding in any of the regions, in contrast to a significant main effect upon both leptin and adiponectin levels in the blood. However, a MANCOVA of the data controlling for leptin and adiponectin as co-variables identified a significant effect of glucose and fructose treatment for five weeks upon the [(35)S]GTPγS response in the ventromedial hypothalamus, a region of importance for regulation of appetite.
It is concluded from this pilot study that palatable solutions do not produce overt changes in brain CB1 receptor functionality, although subtle changes in discrete brain regions may occur.
高果糖摄入不仅会导致胰岛素敏感性和血管功能的外周变化，还会引起多个脑区的中枢变化。鉴于内源性大麻素系统在能量摄入控制中的作用，我们进行了一项初步研究，以确定高果糖饮食是否会导致脑CB1受体功能发生变化。
让自由摄取普通食物的雄性大鼠饮用自来水、葡萄糖溶液或果糖溶液。通过放射自显影法测量CB1受体功能，即激动剂CP5,940产生的[(35)S]GTPγS结合增加量。
研究了七个脑区：前额叶皮质、尾状核 - 壳核、海马CA1 - CA3、齿状回、杏仁核以及背内侧和腹内侧下丘脑。对每个脑区进行的双向稳健Wilcoxon分析表明，与血液中瘦素和脂联素水平受到显著主效应不同，饮食处理在任何脑区对激动剂刺激的[(35)S]GTPγS结合均未产生显著主效应。然而，以瘦素和脂联素作为协变量对数据进行的多变量协方差分析发现，葡萄糖和果糖处理五周对腹内侧下丘脑（对食欲调节很重要的一个区域）的[(35)S]GTPγS反应有显著影响。
从这项初步研究得出的结论是，美味溶液不会导致脑CB1受体功能发生明显变化，尽管在离散的脑区可能会发生细微变化。
