Ogawa Y, Adachi Y, Hong S S, Yagi T
Department of Oral Pathology, Osaka University Faculty of Dentistry, Japan.
Calcif Tissue Int. 1989 Jan;44(1):46-60. doi: 10.1007/BF02556239.
Effects of 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) on the dentinogenesis of the rat maxillary incisor were microradiographically examined 96 hours after a single subcutaneous injection. HEBP produced hypomineralized incremental bands in the enamel (HPOe) and in the dentine (outer HPO1 and inner HPO2) in a dose-dependent manner. In the labial dentine, both HPO1 and HPO2 did not extend apically beyond the apical limit of the HPOe and were thus located in the circumpulpal dentine. The two lesions in the labial dentine fully developed with a dosage of 10 mg/ P/kg or larger, and with 15 mg P/kg or larger, gross hypoplastic lesions developed. Therefore, histological analysis at shorter postinjection intervals was carried out on the labial dentine with 10 mg P/kg of HEBP. The HPO1 appeared along the dentine-predentine junction by 24 hours and was characterized by reduced numbers of inorganic crystals and 10 nm particles which invested the collagen fibrils. Therefore, the HPO1 was judged to be produced by the disturbance of transformation of the predentine to dentine. The precursor lesion of HPO2 appeared in the proximal predentine by 4 hours, and was characterized by reduced number of collagen fibrils and unusually coarse interfibrillar stippled material. This lesion migrated to the dentine by 96 hours and became the HPO2, which was characterized by reduced number of collagen fibrils. Individual collagen fibrils in the HPO2 were invested by as many inorganic crystals and 10 nm particles as in the normal dentine. The HPO2 is considered to be produced by the disturbance of organic matrix formation.
在单次皮下注射96小时后，通过显微放射照相术检查了1-羟基亚乙基-1,1-二膦酸酯（HEBP）对大鼠上颌切牙牙本质形成的影响。HEBP以剂量依赖性方式在釉质（HPOe）和牙本质（外层HPO1和内层HPO2）中产生矿化不足的生长带。在唇侧牙本质中，HPO1和HPO2均未向根尖延伸超过HPOe的根尖界限，因此位于牙髓周围牙本质中。唇侧牙本质中的这两个病变在剂量为10 mg P/kg或更高时完全发展，而在剂量为15 mg P/kg或更高时，出现明显的发育不全病变。因此，对注射10 mg P/kg HEBP的唇侧牙本质在注射后较短时间间隔进行了组织学分析。HPO1在24小时时沿牙本质-前期牙本质交界处出现，其特征是无机晶体数量减少以及包绕胶原纤维的10 nm颗粒减少。因此，判断HPO1是由前期牙本质向牙本质转化的紊乱所产生。HPO2的前期病变在4小时时出现在近端前期牙本质中，其特征是胶原纤维数量减少以及纤维间点状物质异常粗大。该病变在96小时时迁移至牙本质并成为HPO2，其特征是胶原纤维数量减少。HPO2中的单个胶原纤维与正常牙本质一样被许多无机晶体和10 nm颗粒包绕。HPO2被认为是由有机基质形成的紊乱所产生。
