Lundell Anna-Carin, Johansen Susanne, Adlerberth Ingegerd, Wold Agnes E, Hesselmar Bill, Rudin Anna
Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Göteborg 405 30, Sweden;
Pediatric Clinic, Skaraborg Hospital, Lidköping, Lidköping 531 85, Sweden;
J Immunol. 2014 Jul 15;193(2):510-8. doi: 10.4049/jimmunol.1302990. Epub 2014 Jun 13.
Delayed maturation of the immune system has been proposed to be a risk factor for development of allergy, but B cell maturation in relation to allergic disease has not been examined. B cells lose CD5 and acquire CD27 during maturation from immature via mature/naive to Ig-secreting cells and memory cells. We sought to investigate B cell maturation in relation to development of allergic disease and sensitization in the FARMFLORA birth cohort including 65 Swedish children. Total B cell numbers, proportions of CD5(+) and CD27(+) B cells, and levels of IgM, IgG, IgA, and IgE were measured in blood on repeated occasions from birth to 36 mo of age, and related to allergic disease and sensitization at 18 and 36 mo of age with multivariate discriminant analysis. We also compared the expression of CD24 and CD38 within CD5(+) and CD5(neg) B cells in children and in adults. We found that infants with a high proportion of CD5(+) B cells at birth and at 1 mo of age had an increased risk for having allergic disease at 18 and 36 mo of life. Further, the proportions of CD5(+) B cells at 1 mo of age were inversely correlated with total IgG levels at 18 and 36 mo of age. The majority of the CD5(+) B cells were of a CD24(hi/+)CD38(hi/+) immature/naive phenotype at birth (97%), 7 y of age (95%), and in adults (86%). These results suggest that development of allergic disease is preceded by an immaturity in neonatal B cell phenotype.
免疫系统成熟延迟被认为是过敏发生的一个风险因素，但与过敏性疾病相关的B细胞成熟情况尚未得到研究。B细胞在从未成熟经由成熟/幼稚细胞到分泌Ig的细胞和记忆细胞的成熟过程中会丢失CD5并获得CD27。我们试图在包括65名瑞典儿童的FARMFLORA出生队列中研究与过敏性疾病发生和致敏相关的B细胞成熟情况。从出生到36月龄多次测量血液中的总B细胞数量、CD5(+)和CD27(+) B细胞比例以及IgM、IgG、IgA和IgE水平，并通过多变量判别分析将其与18和36月龄时的过敏性疾病和致敏情况相关联。我们还比较了儿童和成人CD5(+)和CD5(-) B细胞中CD24和CD38的表达。我们发现，出生时和1月龄时CD5(+) B细胞比例高的婴儿在18和36月龄时患过敏性疾病的风险增加。此外，1月龄时CD5(+) B细胞比例与18和36月龄时的总IgG水平呈负相关。大多数CD5(+) B细胞在出生时（97%）、7岁时（95%）和成人中（86%）具有CD24(hi/+)CD38(hi/+)未成熟/幼稚表型。这些结果表明，过敏性疾病的发生之前存在新生儿B细胞表型的不成熟。
