Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil; Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, Brazil.
Exp Cell Res. 2014 Aug 1;326(1):103-11. doi: 10.1016/j.yexcr.2014.05.023. Epub 2014 Jun 12.
Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal arterial segments. However, tissue engineering may be an option to yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural tissue architecture to use in tissue engineering, when the absence of ideal autologous veins reduces surgical options. The goal of this study was to evaluate different chemical induced decellularization protocols of the inferior vena cava of rabbits. They were decellularized with Triton X100 (TX100), sodium dodecyl sulfate (SDS) or sodium deoxycholate (DS). Afterwards, we assessed the remaining extracellular matrix (ECM) integrity, residual toxicity and the biomechanical resistance of the scaffolds. Our results showed that TX100 was not effective to remove the cells, while protocols using SDS 1% for 2h and DS 2% for 1h, efficiently removed the cells and were better characterized. These scaffolds preserved the original organization of ECM. In addition, the residual toxicity assessment did not reveal statistically significant changes while decellularized scaffolds retained the equivalent biomechanical properties when compared with the control. Our results concluded that protocols using SDS and DS were effective at obtaining decellularized scaffolds, which may be useful for blood vessel tissue engineering.
临床经验表明，在治疗下肢动脉疾病时，使用合成移植物处理腘动脉以下的动脉段效果不佳。然而，组织工程可能是一种产生替代生物相容性新血管的方法。因此，生物去细胞化支架可以为组织工程提供天然的组织结构，当理想的自体静脉减少手术选择时。本研究的目的是评估不同的化学诱导去细胞化方案兔下腔静脉。用 Triton X100 (TX100)、十二烷基硫酸钠 (SDS) 或脱氧胆酸钠 (DS) 对其进行去细胞化处理。然后，我们评估了支架的剩余细胞外基质 (ECM) 完整性、残留毒性和生物力学阻力。结果表明，TX100 不能有效去除细胞，而使用 1% SDS 处理 2 小时和 2% DS 处理 1 小时的方案能有效去除细胞，并具有更好的特性。这些支架保留了 ECM 的原始组织。此外，残留毒性评估显示，去细胞化支架没有明显的统计学变化，同时保留了与对照组相当的生物力学性能。我们的研究结果表明，SDS 和 DS 的使用方案可有效获得去细胞化支架，这可能对血管组织工程有用。
