MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Biomaterials. 2014 Sep;35(27):7679-89. doi: 10.1016/j.biomaterials.2014.05.047. Epub 2014 Jun 12.
Inspired by the adhesion strategy of marine mussels, self-polymerization of dopamine under alkaline condition has been proven to be a simple and effective method for surface modification of biomaterials. However, this method still has many drawbacks, such as the use of alkaline aqueous medium, low poly(dopamine) deposition rate, and inefficient utilization of dopamine, which greatly hinder its practical application. In the present study, we demonstrate that electropolymerization of dopamine is a facile and versatile approach to surface tailoring of metallic cardiovascular stents, such as small and complex-shaped coronary stent. Electropolymerization of dopamine leads to the formation of a continuous and smooth electropolymerized poly(dopamine) (ePDA) coating on the substrate surface. This electrochemical method exhibits a higher deposition rate and is more efficient in dopamine utilization compared with the typical self-polymerization method. The ePDA coating facilitates the immobilization of biomolecules onto substrates to engineer biomimetic microenvironments. In vitro and in vivo experiments demonstrate that ePDA coating functionalized with vascular endothelial growth factor can greatly enhance the desired cellular responses of endothelial cells and prevent the neointima formation after stent implantation. The proposed methodology may find applications in the area of metallic surface engineering, especially for the cardiovascular stents and potentially all biomedical devices with electroconductive surface as well.
受海洋贻贝粘附策略的启发，在碱性条件下多巴胺的自聚合已被证明是一种对生物材料表面进行改性的简单而有效的方法。然而，这种方法仍然存在许多缺点，例如使用碱性水介质、低聚多巴胺沉积速率和多巴胺利用效率低，这极大地阻碍了其实际应用。在本研究中，我们证明了多巴胺的电化学聚合是一种简便且通用的方法，可用于对金属心血管支架（如小而复杂形状的冠状动脉支架）进行表面修饰。多巴胺的电化学聚合导致在基底表面上形成连续且光滑的电聚合聚多巴胺（ePDA）涂层。与典型的自聚合方法相比，这种电化学方法具有更高的沉积速率和更高的多巴胺利用效率。ePDA 涂层有利于将生物分子固定在基底上，以构建仿生微环境。体外和体内实验表明，血管内皮生长因子功能化的 ePDA 涂层可大大增强内皮细胞的所需细胞反应，并防止支架植入后的新内膜形成。所提出的方法可能在金属表面工程领域得到应用，特别是对于心血管支架以及潜在的所有具有导电表面的生物医学设备。
