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脑膜炎球菌1类和3类外膜蛋白的纯化、溴化氰裂解及氨基末端测序

Purification, cyanogen bromide cleavage, and amino terminus sequencing of class 1 and class 3 outer membrane proteins of meningococci.

作者信息

Poolman J T, Timmermans H A, Teerlink T, Seid R C

机构信息

Department of Bacterial Vaccine Development, National Institute for Public Health and Environmental Protection (RIVM), Bilthoven, The Netherlands.

出版信息

Infect Immun. 1989 Mar;57(3):1005-7. doi: 10.1128/iai.57.3.1005-1007.1989.

DOI:10.1128/iai.57.3.1005-1007.1989
PMID:2492968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313215/
Abstract

Meningococcal class 1 and 3 outer membrane proteins (OMPs) were subjected to cyanogen bromide treatment. The class 3 OMP was found to be resistant to cyanogen bromide, while the class 1 OMP was cut into two main fragments of 25 and 17 kilodaltons. The N-terminal sequences were determined for class 1 and class 3 proteins, which exhibit similarities to one another and to OMP I of gonococci. The C-terminal class 1 OMP fragment bound the bactericidal monoclonal antibodies tested.

摘要

脑膜炎球菌1类和3类外膜蛋白（OMPs）接受了溴化氰处理。发现3类OMP对溴化氰有抗性，而1类OMP被切割成两个主要片段，分子量分别为25千道尔顿和17千道尔顿。测定了1类和3类蛋白的N端序列，它们彼此之间以及与淋球菌的OMP I具有相似性。1类OMP的C端片段与所测试的杀菌单克隆抗体结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/4b161d7a8422/iai00063-0356-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/bc7921ecf5f0/iai00063-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/19ef2a36fd9a/iai00063-0356-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/4b161d7a8422/iai00063-0356-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/bc7921ecf5f0/iai00063-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/19ef2a36fd9a/iai00063-0356-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0c7/313215/4b161d7a8422/iai00063-0356-c.jpg

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Enrichment of subgingival microflora on human serum leading to accumulation of Bacteroides species, Peptostreptococci and Fusobacteria.人类血清中龈下微生物群的富集导致拟杆菌属、消化链球菌和梭杆菌的积累。
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Neisseria meningitidis group B serosubtyping using monoclonal antibodies in whole-cell ELISA.
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Infect Immun. 1990 May;58(5):1355-9. doi: 10.1128/iai.58.5.1355-1359.1990.
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