Rodriguez C, Taminiau B, Avesani V, Van Broeck J, Delmée M, Daube G
Food Science Department, Faculty of Veterinary Medicine, University of Liège, B43bis, Sart-Tilman, 4000 Liège, Belgium.
Food Science Department, Faculty of Veterinary Medicine, University of Liège, B43bis, Sart-Tilman, 4000 Liège, Belgium.
Food Microbiol. 2014 Sep;42:166-71. doi: 10.1016/j.fm.2014.03.021. Epub 2014 Apr 2.
Clostridium difficile has been isolated from food animals and meat, specially ground pork and ground beef. The recovered isolates were closely related to C. difficile human strains, indicating that animals and food are possible transmission routes of human C. difficile infection. The main objective of this study was to characterize C. difficile isolates from retail meat and to compare with human isolates recovered from hospital patients in Belgium. Raw meat (beef and pork) was obtained from the retail trade. C. difficile was recovered from 2.3% of the beef samples and from 4.7% of the pork samples. A total of 4 different PCR-ribotypes were identified with a large percentage of types 078 and 014. Resistance to moxifloxacin and erythromycin was detected. The multi-locus sequence typing (MLST) analysis showed that meat and human isolates cluster in the same lineage. This study reveals the presence of toxigenic C. difficile in retail meat in Belgium with predominance PCR-ribotypes 078 and 014, which are among the four most prevalent ribotypes of C. difficile isolated from humans in Europe.
艰难梭菌已从食用动物和肉类中分离出来，特别是绞碎的猪肉和牛肉。回收的分离株与艰难梭菌人类菌株密切相关，表明动物和食物可能是人类艰难梭菌感染的传播途径。本研究的主要目的是对零售肉类中的艰难梭菌分离株进行表征，并与从比利时医院患者中分离出的人类分离株进行比较。生肉（牛肉和猪肉）取自零售行业。从2.3%的牛肉样本和4.7%的猪肉样本中分离出了艰难梭菌。共鉴定出4种不同的PCR核糖型，其中078型和014型占很大比例。检测到对莫西沙星和红霉素的耐药性。多位点序列分型（MLST）分析表明，肉类和人类分离株聚集在同一谱系中。这项研究揭示了比利时零售肉类中存在产毒艰难梭菌，主要PCR核糖型为078和014，这两种核糖型是欧洲从人类中分离出的四种最常见的艰难梭菌核糖型之一。
