Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via Salvemini 1, 06126 Perugia, Italy.
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
Int J Environ Res Public Health. 2021 Oct 29;18(21):11368. doi: 10.3390/ijerph182111368.
In the last decade, the incidence and severity of infections (CDIs) in humans have been increasing and community-associated infections have been described. For these reasons, the interest in in food and in food animals has increased, suggesting other possible sources of acquisition. This study evaluated the presence of on pig carcasses at the slaughterhouse and in pork products in Central Italy. The contamination rate on pig carcasses was 4/179 (2.3%). Regarding food samples, a total of 216 pork products were tested (74 raw meat preparations and 142 ready-to-eat food samples made by cured raw meat). The real-time PCR screening was positive for 1/74 raw meat preparation (1.35%) and for 1/142 ready-to-eat food samples (0.7%) was isolated only from the raw meat preparation (pork sausage). All the isolated strains were toxigenic and susceptible to all the tested antibiotics. Strains isolated from carcass samples displayed A+B+CDTa+CDTb+ profile, were toxinotype IV and belonged to the same ribotype arbitrary named TV93, while the one isolated from food samples displayed A+B+CDTa-CDTb- profile and it was not possible to determine ribotype and toxinotype, because it was lost after freeze storage. It was concluded that the prevalence of in the pork supply chain is very low.
在过去的十年中，人类感染（CDI）的发病率和严重程度一直在增加，并且已经描述了社区相关性感染。由于这些原因，人们对食品和食品动物中的 的兴趣增加了，这表明可能还有其他获得 的来源。本研究评估了意大利中部屠宰场猪胴体和猪肉产品中 的存在情况。猪胴体的污染率为 4/179（2.3%）。关于食品样本，共测试了 216 个猪肉产品（74 个生肉制剂和 142 个由生肉腌制制成的即食食品样本）。实时 PCR 筛查显示 1/74 个生肉制剂（1.35%）和 1/142 个即食食品样本（0.7%）呈阳性 仅从生肉制剂（猪肉香肠）中分离出来。所有分离株均具有毒力，且对所有测试的抗生素均敏感。从胴体样本中分离出的菌株显示 A+B+CDTa+CDTb+ 型，毒素型为 IV，属于同一随机命名为 TV93 的核糖体型，而从食品样本中分离出的菌株显示 A+B+CDTa-CDTb- 型，无法确定核糖体型和毒素型，因为它在冷冻储存后丢失。结论是猪肉供应链中 的流行率非常低。
