Jones T H, Nattress F M, Dilts B, Olsen D, Muehlhauser V
Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada.
Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada.
Food Microbiol. 2014 Sep;42:225-31. doi: 10.1016/j.fm.2014.04.001. Epub 2014 Apr 19.
The numbers of coliforms, Escherichia coli, F-RNA coliphages, bovine enteric calicivirus (BEC) and rotavirus (RV) and presence of non-O157 shiga toxigenic E. coli (STEC) were determined on commercial vacuum packaged beef subprimals at the retail level from swabs obtained from the entire surfaces of 150 cuts that originated from federally and provincially registered plants. The prevalence and log mean numbers of E. coli were higher in provincially registered plants than in federally registered plants; 64% vs 20%, respectively, and -0.3 vs -1.22 log cfu/100 cm(2), respectively. In contrast, the prevalence and mean log numbers of F-RNA coliphages were lower for the provincially registered plants than for the federally registered plants; 31% vs 68% and -0.86 vs -0.13 log cfu/100 cm(2), respectively. One E. coli sample tested positive for stx2 and eae. F-RNA coliphages associated with human origin (GII/GIII) were detected in 12% and 30% of samples that originated from provincially and federally registered plants, respectively. RV RNA was detected in 4% of samples while BEC RNA was not detected. Although the infectivity of RV is unknown, the presence of viable F-RNA coliphages suggests that consumers could potentially be at risk when consuming undercooked meat that is contaminated with RV.
对来自联邦和省级注册工厂的150块切块的整个表面拭子进行检测，以确定零售层面商业真空包装牛肉二级切块中的大肠菌群、大肠杆菌、F-RNA噬菌体、牛肠道杯状病毒（BEC）和轮状病毒（RV）数量，以及非O157产志贺毒素大肠杆菌（STEC）的存在情况。省级注册工厂中大肠杆菌的患病率和对数平均数量高于联邦注册工厂；分别为64%和20%，以及分别为-0.3和-1.22 log cfu/100 cm²。相比之下，省级注册工厂中F-RNA噬菌体的患病率和平均对数数量低于联邦注册工厂；分别为31%和68%，以及分别为-0.86和-0.13 log cfu/100 cm²。一份大肠杆菌样本stx2和eae检测呈阳性。分别在来自省级和联邦注册工厂的12%和30%的样本中检测到与人源相关的F-RNA噬菌体（GII/GIII）。在4%的样本中检测到RV RNA，而未检测到BEC RNA。尽管RV的传染性未知，但存活的F-RNA噬菌体的存在表明，消费者食用受RV污染的未煮熟肉类时可能存在风险。
