Feld Geoffrey K, Frank Matthias
Biosciences and Biotechnology Division, Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.
Physics Division, Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.
Curr Opin Struct Biol. 2014 Aug;27:69-78. doi: 10.1016/j.sbi.2014.05.002. Epub 2014 Jun 12.
Determining the three-dimensional structures and dynamics of membrane proteins remains one of the great challenges of modern biology. The recent availability of X-ray free electron laser (XFEL) light sources has opened the door to a new and revolutionary approach to performing X-ray analysis of these important biomolecules. Recent advances in sample delivery, data reduction, and phasing have enabled the high-resolution structural probing of membrane proteins at room temperature. While considerable challenges remain, the recent developments described in this review may ultimately provide structural biologists with powerful tools for obtaining unprecedented atomic-scale and dynamic visualization of membrane proteins at near-physiological conditions.
确定膜蛋白的三维结构和动力学仍然是现代生物学面临的重大挑战之一。最近X射线自由电子激光(XFEL)光源的出现为对这些重要生物分子进行X射线分析开辟了一种全新的革命性方法。样品输送、数据处理和相位分析方面的最新进展使得在室温下对膜蛋白进行高分辨率结构探测成为可能。尽管仍然存在诸多挑战,但本综述中描述的最新进展最终可能为结构生物学家提供强大的工具,以便在接近生理条件下获得膜蛋白前所未有的原子尺度和动态可视化信息。