Keles Didem, Arslan Baha, Terzi Cem, Tekmen Isil, Dursun Ezgi, Altungoz Oguz, Oktay Gulgun
Dokuz Eylul University, School of Medicine, Department of Medical Biochemistry, 35340 Izmir, Turkey.
Dokuz Eylul University, School of Medicine, Department of Surgery, 35340 Izmir, Turkey.
Clin Biochem. 2014 Sep;47(13-14):1265-71. doi: 10.1016/j.clinbiochem.2014.06.004. Epub 2014 Jun 13.
Matrix metalloproteinase-7 is capable of degrading several ECM and non-ECM molecules and contributes to colorectal cancer progression and metastasis. Here, we examined the significance of MMP-7 in colorectal tumors by detecting active and latent MMP-7 levels and localization of its caseinolytic activity.
We investigated expression levels, localization, and proteolytic activity of MMP-7 and local caseinolytic activity in colorectal tumor and paired normal tissues by using real time PCR, casein zymography, immunohistochemistry and in situ casein zymography, respectively. In addition the results were compared with clinicopathological variables.
Real time PCR and immunohistochemistry showed that MMP-7 expressions were higher in colorectal tumor tissues than in normal tissues. Also, mRNA expressions of MMP-7 were positively correlated with tumor and pathological stages and negatively correlated with age. Furthermore, MMP-7 mRNA expression had a sensitivity of 81.3% and a specificity of 81.2% at a cut-off value of 0.0006, making it a potential marker for diagnosis of colorectal cancer. According to casein zymography, pro- and active MMP-7 levels were also elevated in tumor tissues. In addition, we assessed local caseinolytic activity using in situ casein zymography. Increased immunoreactivity of MMP-7 and local caseinolytic activity were found in neoplastic cells but not in stromal cells.
We emphasized the significant role of MMP-7 in diagnosis and progression and/or development of colorectal cancer.
基质金属蛋白酶-7能够降解多种细胞外基质（ECM）和非ECM分子，并促进结直肠癌的进展和转移。在此，我们通过检测活性和潜伏性MMP-7水平及其酪蛋白溶解活性的定位，研究了MMP-7在结直肠肿瘤中的意义。
我们分别使用实时PCR、酪蛋白酶谱法、免疫组织化学和原位酪蛋白酶谱法，研究了MMP-7在结直肠肿瘤及配对正常组织中的表达水平、定位和蛋白水解活性以及局部酪蛋白溶解活性。此外，将结果与临床病理变量进行了比较。
实时PCR和免疫组织化学显示，MMP-7在结直肠肿瘤组织中的表达高于正常组织。此外，MMP-7的mRNA表达与肿瘤和病理分期呈正相关，与年龄呈负相关。此外，MMP-7 mRNA表达在截断值为0.0006时，灵敏度为81.3%，特异性为81.2%，使其成为结直肠癌诊断的潜在标志物。根据酪蛋白酶谱法，肿瘤组织中前体和活性MMP-7水平也升高。此外，我们使用原位酪蛋白酶谱法评估了局部酪蛋白溶解活性。在肿瘤细胞中发现MMP-7的免疫反应性增加和局部酪蛋白溶解活性增加，而在基质细胞中未发现。
我们强调了MMP-7在结直肠癌诊断、进展和/或发展中的重要作用。
