Bengi Gösel, Keles Didem, Topalak Ömer, Yalçin Mustafa, Kiyak Rabia, Oktay Gülgün
Department of Gastroenterology, Dokuz Eylul University Hospital, izmir, Turkey.
Department of Biochemistry, Dokuz Eylul University Hospital, izmir, Turkey.
Euroasian J Hepatogastroenterol. 2015 Jul-Dec;5(2):74-79. doi: 10.5005/jp-journals-10018-1138. Epub 2016 Jul 9.
We aimed to investigate the relationship of expression of matrix metalloproteinase-7 (MMP-7), tissue inhibitor of metalloproteinase-1 (TIMP-1) and cyclooxygenase-2 (COX-2) in colon cancer and its predecessor colon polyp.
This study included 29 patients with colon polyp, 19 patients with colon cancer and 65 healthy control subjects. The expressions of MMP-7, TIMP-1 and COX-2 were investigated by real time-polymerase chain reaction (RT-PCR).
The expressions of TIMP-1, COX-2 and MMP-7 levels were significantly higher in polyp tissue compared to normal tissue (p = 0.024, p < 0.001, p = 0.009, respectively). Expression of TIMP-1, COX-2 and MMP-7 in cancer tissues were higher than both normal tissue and polyp tissue (p = 0.009 and p = 0.001; p < 0.001 and p < 0.001; p = 0.029 and p = 0.008, respectively). In the cancer group, no significant relationship was detected between metastasis and MMP-7, TIMP-1 and COX-2 expressions (p > 0.05). In the polyp tissues, no significant relationship was detected between the histologic type and size of polyps and MMP-7, TIMP-1 and COX-2 levels (p > 0.05). The areas under the receiver operating characteristic (ROC) curve for the cancer group were 0.821 for TIMP-1, 0.888 for COX-2, and 0.880 for MMP-7 (p = 0 < 0.001).
A role and implication of expressions of MMP-7, COX-2 and TIMP-1 in colon cancer is predicted.
Bengi G, Keles D, Topalak Ö, Yalçin M, Kiyak R, Oktay G. Expressions of TIMP-1, COX-2 and MMP-7 in Colon Polyp and Colon Cancer. Euroasian J Hepato-Gastroenterol 2015;5(2):74-79.
我们旨在研究基质金属蛋白酶-7（MMP-7）、金属蛋白酶组织抑制剂-1（TIMP-1）和环氧化酶-2（COX-2）在结肠癌及其癌前病变结肠息肉中的表达关系。
本研究纳入29例结肠息肉患者、19例结肠癌患者及65例健康对照者。采用实时聚合酶链反应（RT-PCR）检测MMP-7、TIMP-1和COX-2的表达。
与正常组织相比，息肉组织中TIMP-1、COX-2和MMP-7水平的表达显著更高（分别为p = 0.024、p < 0.001、p = 0.009）。癌组织中TIMP-1、COX-2和MMP-7的表达高于正常组织和息肉组织（分别为p = 0.009和p = 0.001；p < 0.001和p < 0.001；p = 0.029和p = 0.008）。在癌症组中，未检测到转移与MMP-7、TIMP-1和COX-2表达之间存在显著关系（p > 0.05）。在息肉组织中，未检测到息肉的组织学类型和大小与MMP-7、TIMP-1和COX-2水平之间存在显著关系（p > 0.05）。癌症组中，TIMP-1的受试者工作特征（ROC）曲线下面积为0.821，COX-2为0.888，MMP-7为0.880（p = 0 < 0.001）。
预测MMP-7、COX-2和TIMP-
1的表达在结肠癌中具有一定作用和意义。
Bengi G, Keles D, Topalak Ö, Yalçin M, Kiyak R, Oktay G. TIMP-1、COX-2和MMP-7在结肠息肉和结肠癌中的表达。《欧亚肝脏胃肠病学杂志》2015；5(2):74 - 79。
