Takada Tadao, Yamaguchi Kosato, Tsukamoto Suguru, Nakamura Mitsunobu, Yamana Kazushige
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
Analyst. 2014 Aug 21;139(16):4016-21. doi: 10.1039/c4an00493k.
Here we study the binding behavior of perylenediimide () derivatives to a hydrophobic pocket created inside DNA and their photochemical properties capable of designing a light-up fluorescent sensor for short single-stranded DNA or RNA. The perylenediimide derivative with alkoxy groups () suppressing electron transfer quenching was examined. The bound randomly to DNA showed negligible fluorescence due to the aggregation-induced quenching, whereas the bound to the pocket as a monomeric form showed more than 100-fold fluorescence enhancement. Switching the binding states of the corresponded to a change in the fluorescence response for the hybridization event, which allowed us to design a fluorescent sensor of nucleic acids with a nanomolar detection limit.
在此，我们研究了苝二酰亚胺（）衍生物与DNA内部形成的疏水口袋的结合行为，以及它们能够设计用于短单链DNA或RNA的发光荧光传感器的光化学性质。研究了具有烷氧基（）抑制电子转移猝灭的苝二酰亚胺衍生物。随机结合到DNA上的由于聚集诱导猝灭而显示出可忽略不计的荧光，而以单体形式结合到口袋中的则显示出超过100倍的荧光增强。相应物结合状态的切换对应于杂交事件荧光响应的变化，这使我们能够设计出具有纳摩尔检测限的核酸荧光传感器。
