Xiao Songshu, Liao Shan, Zhou Yanhong, Jiang Bin, Li Yueran, Xue Min
Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
Molecular Genetics Laboratory, Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China.
Oncol Lett. 2014 Jun;7(6):1889-1894. doi: 10.3892/ol.2014.2023. Epub 2014 Apr 2.
Cervical carcinoma is the second most prevalent malignancy in females worldwide. The crucial etiologic factors involved in the development of cervical carcinoma include infection with papillomavirus, and the structural or functional mutation of oncogenes and tumor suppressor genes. The abnormal change of octamer transcription factor 1 (OCT1) is associated with tumor progression and a poor patient survival rate. However, little is known regarding the effect of OCT1 in cervical cancer. In the present study, flow cytometry, western blot analysis and quantitative polymerase chain reaction (qPCR) were peformed to identify differentially expressed OCT1 in cervical cancer tissue and adjacent non-cancerous tissues. The normalized OCT1 gene expression in cervical cancer was 5.98 times higher compared with the adjacent non-cancerous tissues. Western blot analysis and flow cytometry assessed the levels of OCT1 protein. The results of these two differential techniques showed that the protein expression level of OCT1 was greater in cervical cancer tissues, which corresponded with the qPCR results. Finally, as OCT1 is a potential target gene for microRNA (miR)-1467, -1185, -4493 and -3919, their expression levels were analyzed in cervical cancer tissues and adjacent non-cancerous tissues; they were downregulated by ~45% in the cervical cancer samples. The results of the present study showed that OCT1 is highly expressed in cervical cancer tissues and indicated that OCT-1 may be significant in cervical cancer.
宫颈癌是全球女性中第二大常见恶性肿瘤。宫颈癌发生过程中涉及的关键病因包括乳头瘤病毒感染以及癌基因和肿瘤抑制基因的结构或功能突变。八聚体转录因子1（OCT1）的异常变化与肿瘤进展及患者低生存率相关。然而，关于OCT1在宫颈癌中的作用知之甚少。在本研究中，采用流式细胞术、蛋白质印迹分析和定量聚合酶链反应（qPCR）来鉴定宫颈癌组织和相邻非癌组织中差异表达的OCT1。宫颈癌中OCT1基因的标准化表达水平比相邻非癌组织高5.98倍。蛋白质印迹分析和流式细胞术评估了OCT1蛋白的水平。这两种差异技术的结果显示，OCT1蛋白表达水平在宫颈癌组织中更高，这与qPCR结果一致。最后，由于OCT1是微小RNA（miR）-1467、-1185、-4493和-3919的潜在靶基因，分析了它们在宫颈癌组织和相邻非癌组织中的表达水平；在宫颈癌样本中它们下调了约45%。本研究结果表明，OCT1在宫颈癌组织中高表达，提示OCT-1在宫颈癌中可能具有重要意义。
