[从活跃链霉菌中克隆诺西肽抗性基因]
[Cloning of a nosiheptide-resistant gene from Streptomyces actuosus].
作者信息
Dary A, Simonet B, Simonet J M, Decaris B
机构信息
Laboratoire de Génétique et Microbiologie, Faculté des sciences, Université de Nancy-I.
出版信息
C R Acad Sci III. 1989;308(2):35-41.
PMID:2493312
Abstract
The cloning of the nosiheptide resistance gene (nos) from S. actuosus, which produces this antibiotic, in plasmid pS10147 was carried out in S. lividans. The nosipheptide resistant clones contained the plasmid pNS1. It deletion derivative plasmid, pNS2, confers nosiheptide resistance to both S. ambofaciens and S. lividans.
摘要
在产抗生素的活跃链霉菌中克隆诺西肽抗性基因（nos），并将其克隆到质粒pS10147中，该过程在淡紫灰链霉菌中进行。诺西肽抗性克隆含有质粒pNS1。其缺失衍生质粒pNS2赋予栖土曲霉和淡紫灰链霉菌诺西肽抗性。
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