来自活跃链霉菌ATCC 25421的诺西肽抗性基因的分子克隆。

Molecular cloning of the nosiheptide resistance gene from Streptomyces actuosus ATCC 25421.

作者信息

Dosch D C, Strohl W R, Floss H G

机构信息

Department of Microbiology, Ohio State University, Columbus 43210.

出版信息

Biochem Biophys Res Commun. 1988 Oct 14;156(1):517-23. doi: 10.1016/s0006-291x(88)80872-6.

DOI:10.1016/s0006-291x(88)80872-6

PMID:2845982

Abstract

An 8.5 kb BamHI DNA fragment conferring resistance to nosiheptide, a peptide antibiotic of the 'thiostrepton group', was cloned from Streptomyces actuosus ATCC 25421 in Streptomyces lividans 1326. Two BamHI fragments of S. actuosus, the 8.5 kb fragment and an additional 3.0 kb fragment, hybridized with a thiostrepton resistance gene probe (pIJ30). The 8.5 kb fragment showed a relatively low degree of homology with the thiostrepton resistance gene. The restriction map of the nosiheptide resistance gene isolated here was significantly different from the map of the thiostrepton resistance gene previously published.

摘要

从活跃链霉菌ATCC 25421中克隆出一个8.5 kb的BamHI DNA片段，该片段赋予对“硫链丝菌素类”肽抗生素诺西肽的抗性，此片段被克隆到淡紫灰链霉菌1326中。活跃链霉菌的两个BamHI片段，即8.5 kb片段和另一个3.0 kb片段，与硫链丝菌素抗性基因探针（pIJ30）杂交。8.5 kb片段与硫链丝菌素抗性基因显示出相对较低的同源性。此处分离出的诺西肽抗性基因的限制酶切图谱与先前发表的硫链丝菌素抗性基因图谱显著不同。