Allain P, Turcant A, Prémel-Cabic A
Laboratoire de Pharmacologie, C.H.U., Angers, France.
Clin Chem. 1989 Mar;35(3):469-70.
For automated determination of theophylline and valproic acid by use of Ames' fluoroimmunoassays we used a high-pressure liquid-chromatographic system consisting of a pump, a robotic unit (Gilson 231/401, to prepare and inject the samples into the flowing carrier), and a fluorometer with a 10-microL flow cell. Results correlated well with those of conventional liquid- and gas-chromatography (r less than 0.96). The between-run CV is about 5%. In comparison with the manual method, the volume of reagents (and thus the cost per analysis) was decreased by eightfold.
为了利用艾姆斯公司的荧光免疫分析法自动测定茶碱和丙戊酸，我们使用了一个高压液相色谱系统，该系统由一台泵、一个自动装置（吉尔森231/401，用于制备样品并将其注入流动载体）和一台配备10微升流通池的荧光计组成。结果与传统液相色谱法和气相色谱法的结果相关性良好（r小于0.96）。批间变异系数约为5%。与手工方法相比，试剂用量（从而每次分析的成本）减少了八倍。
