a Pharmacognosy Department , Faculty of Pharmacy, Cairo University , Kasr El-Aini 11562 , Egypt.
Nat Prod Res. 2014;28(19):1549-56. doi: 10.1080/14786419.2014.926353. Epub 2014 Jun 17.
This study attempts to elucidate the secondary metabolite profiles of Ficus lyrata leaves and fruits grown in Egypt. Non-targeted metabolite profiling via ultra performance liquid chromatography (UPLC)-qTOF-MS was used to identify various chemical classes in F. lyrata fruits and leaves (i.e. flavonoids, phenolic acids and fatty acids) analysed by chemometrics. A total of 72 metabolites were evaluated via a UPLC-qTOF-MS-based metabolomic study. Seventeen flavonoids were characterised and tentatively identified with the main constituents being catechins/procyanidins, O- and C-linked flavonoid glycosides. The major procyanidins were dimers and trimers comprising (epi)catechin and (epi)afzelechin units, whereas the predominant flavones were C-glycosides of luteolin and apigenin. Aside from these major flavonoid classes, a group of benzoic acids, caffeoylquinic acids, fatty acid and sphingolipids were also annotated. This study provides the most complete map for polyphenol distribution in F. lyrata leaves and fruits and the basis for future investigation of its fruits nutritional value or possible nutraceutical uses.
本研究试图阐明在埃及生长的榕树叶和果实的次生代谢产物谱。通过超高效液相色谱（UPLC）-qTOF-MS 进行非靶向代谢物分析，并通过化学计量学对榕树叶和果实中的各种化学类群（如类黄酮、酚酸和脂肪酸）进行分析。通过基于 UPLC-qTOF-MS 的代谢组学研究，共评估了 72 种代谢物。鉴定并初步鉴定了 17 种类黄酮，其主要成分是儿茶素/原花青素、O-和 C-连接的类黄酮糖苷。主要的原花青素是由表儿茶素和表阿夫儿茶素单元组成的二聚体和三聚体，而主要的黄酮类是木樨草素和芹菜素的 C-糖苷。除了这些主要的类黄酮类，还注释了一组苯甲酸、咖啡酰奎宁酸、脂肪酸和神经酰胺。本研究提供了榕树叶和果实中多酚分布的最完整图谱，为进一步研究其果实的营养价值或可能的营养保健品用途奠定了基础。
