Farag Nermeen F, Farag Mohamed A, Abdelrahman Enas H, Azzam Shadia M, El-Kashoury El-Sayeda A
a Pharmacognosy Department , Faculty of Pharmacy, Cairo University , Kasr El-Aini Street, P.B. 11562 Cairo , Egypt.
Nat Prod Res. 2015;29(14):1342-9. doi: 10.1080/14786419.2015.1025396. Epub 2015 Mar 26.
Methanol-soluble constituents from the flowers, non-flowering aerial parts and roots of Chrysanthemum pacificum Nakai were analysed via high resolution UPLC-PDA-qTOF-MS followed by chemometrics. Forty-seven chromatographic peaks belonging to various metabolite classes were detected. Most metabolite classes showed qualitative and quantitative differences across parts, with luteolin conjugates being mostly enriched in flowers whereas non-flowering aerial parts contained mostly quercetin and methoxylated flavone conjugates. Root sample ranked the lowest for all flavones and dicaffeoylquinic acids. In contrast, 1,5-di-caffeoylquinic acid levels were found at high levels in flowers and aerial parts reaching 3145 and 1390 μg/g, respectively, suggesting that C. pacificum could serve as a natural resource of this well-recognised anti-hepatotoxic phenolic. Principal component analysis was further used for organs classification in an untargeted manner. This study provides the first map of secondary metabolites distribution in C. pacificum Nakai organs.
通过高分辨率超高效液相色谱-光电二极管阵列-四极杆飞行时间质谱联用仪（UPLC-PDA-qTOF-MS）并结合化学计量学方法，对太平洋菊（Chrysanthemum pacificum Nakai）的花、未开花地上部分和根中的甲醇溶性成分进行了分析。共检测到47个属于不同代谢物类别的色谱峰。大多数代谢物类别在不同部位表现出定性和定量差异，木犀草素共轭物主要富集在花中，而未开花地上部分主要含有槲皮素和甲氧基黄酮共轭物。根样品中所有黄酮和二咖啡酰奎宁酸的含量最低。相比之下，花和地上部分中1,5-二咖啡酰奎宁酸的含量较高，分别达到3145和1390μg/g，这表明太平洋菊可作为这种公认的抗肝毒性酚类物质的天然来源。主成分分析进一步用于以非靶向方式对器官进行分类。本研究提供了太平洋菊器官中次生代谢物分布的首张图谱。
