使用DNA模板化点击化学将长链DNA分子共价、序列特异性地连接到表面。
Covalent, sequence-specific attachment of long DNA molecules to a surface using DNA-templated click chemistry.
作者信息
Abel Gary R, Cao Blessing Huynh, Hein Jason E, Ye Tao
机构信息
Chemistry and Chemical Biology, School of Natural Sciences, University of California, 5200 N. Lake Rd., Merced, CA 95343, USA.
出版信息
Chem Commun (Camb). 2014 Aug 4;50(60):8131-3. doi: 10.1039/c4cc02900c.
PMID:24934265
Abstract
We present a novel method that covalently and sequence-specifically attaches long DNA molecules to a surface that is compatible with high-resolution atomic force microscopy (AFM) imaging. Surfaces prepared with this approach are ideally suited for performing biophysical experiments on single DNA molecules.
摘要
我们提出了一种新方法，该方法能将长链DNA分子以共价且序列特异性的方式连接到与高分辨率原子力显微镜（AFM）成像兼容的表面上。用这种方法制备的表面非常适合对单个DNA分子进行生物物理实验。
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