Dharni Seema, Maurya Anupam, Samad Abdul, Srivastava Santosh Kumar, Sharma Ashok, Patra Dharani Dhar
Agronomy and Soil Science Division, ‡Biotechnology Division, #Chemical Sciences Division, and ⊥Crop Protection Division, CSIR-Central Institute of Medicinal and Aromatic Plants , Lucknow 226015, India.
J Agric Food Chem. 2014 Jul 2;62(26):6138-46. doi: 10.1021/jf5001138. Epub 2014 Jun 23.
A novel strain of Pseudomonas monteilii, PsF84, was isolated from tannery waste soil from Jajmau, Kanpur, India. 16S rRNA gene sequence phylogenetic analysis confirmed the taxonomic affiliation of PsF84 as P. monteilii. An antifungal volatile organic compound (VOC) active against hyphal growth of Fusarium oxysporum (CIMAP-IMI-357464) in vitro was isolated from strain PsF84 by using chromatographic techniques. The molecular formula of the antifungal VOC was deduced to be C₁₄H₂₂O by EI-MS and 1D and 2D NMR spectral analysis. 2,4-Di-tert-butylphenol was found to be effective against an agriculturally important fungus, namely, F. oxysporum, in inhibiting spore germination and hyphal growth. Molecular docking analysis of 2,4-di-tert-butylphenol with β-tubulin further validated the potential of β-tubulin binding in F. oxysporum. Two residues of β-tubulin protein, HIS 118 and THR 117, showed hydrogen binding with ligand. To the authors' knowledge, this is the first report of antifungal VOC (2,4-di-tert-butylphenol) produced by P. monteilii PsF84 that can be a potent inhibitor of β-tubulin of F. oxysporum.
从印度坎普尔贾伊莫的制革厂废土中分离出一种新型蒙特利假单胞菌菌株PsF84。16S rRNA基因序列系统发育分析证实了PsF84在分类学上属于蒙特利假单胞菌。通过色谱技术从菌株PsF84中分离出一种对尖孢镰刀菌（CIMAP - IMI - 357464）体外菌丝生长具有活性的抗真菌挥发性有机化合物（VOC）。通过电子轰击质谱（EI - MS）以及一维和二维核磁共振光谱分析，推断该抗真菌VOC的分子式为C₁₄H₂₂O。发现2,4 - 二叔丁基苯酚对一种重要的农业真菌尖孢镰刀菌有效，可抑制其孢子萌发和菌丝生长。2,4 - 二叔丁基苯酚与β - 微管蛋白的分子对接分析进一步验证了其在尖孢镰刀菌中与β - 微管蛋白结合的潜力。β - 微管蛋白的两个残基HIS 118和THR 117与配体形成氢键。据作者所知，这是关于蒙特利假单胞菌PsF84产生的抗真菌VOC（2,4 - 二叔丁基苯酚）的首次报道，该化合物可能是尖孢镰刀菌β - 微管蛋白的有效抑制剂。
