Felton D, Bergenholtz G, Cox C F
Department of Fixed Prosthodontics, University of North Carolina, School of Dentistry 27599-7450.
J Dent Res. 1989 Mar;68(3):491-5. doi: 10.1177/00220345890680031201.
The purpose of this investigation was to test in five adult monkeys the effects of a glutaraldehyde-containing dentin bonding agent, GLUMA, on bacterial colonization in Class V cavities restored with composite resin. Experimental groups consisted of immediate placement of GLUMA and composite resin as well as placement of GLUMA or Scotchbond (control) in acid-etched cavities that had been left open to the oral environment for 48 hours. Various procedures for pretreatment of the cavities were included. Tissue specimens were prepared for light microscopy for observation of bacterial presence and pulp tissue reactions after eight days and 90 days. Bacteria were not detected in any of the 54 cavities treated with GLUMA regardless of observation period or use of enamel-etching procedure prior to placement of composite resin. When cavities were restored with composite resin without prior GLUMA pretreatment or with Scotchbond, bacteria were present under the majority of restorations at both time intervals. Pulpal inflammation of varying extent and character was seen after eight days in teeth that had been previously infected. At 90 days, pulps showed repair and healing regardless of treatment protocol. Data indicate that GLUMA has a distinct in vivo antibacterial effect that seems to prevent bacterial growth in tooth/restoration interfaces.
本研究的目的是在五只成年猴子身上测试一种含戊二醛的牙本质粘结剂GLUMA对用复合树脂修复的V类洞细菌定植的影响。实验组包括立即放置GLUMA和复合树脂，以及在已向口腔环境开放48小时的酸蚀洞中放置GLUMA或Scotchbond（对照）。包括了各种洞预处理程序。制备组织标本用于光学显微镜观察，以观察八天和90天后细菌的存在情况以及牙髓组织反应。无论观察期如何或在放置复合树脂之前是否使用釉质酸蚀程序，在用GLUMA处理的54个洞中均未检测到细菌。当洞在未事先用GLUMA预处理或用Scotchbond的情况下用复合树脂修复时，在两个时间间隔的大多数修复体下方均存在细菌。在先前已感染的牙齿中，八天后可见不同程度和特征的牙髓炎症。在90天时，无论治疗方案如何，牙髓均显示修复和愈合。数据表明，GLUMA具有明显的体内抗菌作用，似乎可防止细菌在牙齿/修复体界面生长。
