Peng Yong, Guo Cong-Shan, Li Pan-Xia, Fu Zhan-Zhao, Gao Li-Ming, Di Ya, Ju Ya-Kun, Tian Ru, Xue Jia-Jia
Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University, Qinhuangdao, China E-mail :
Asian Pac J Cancer Prev. 2014;15(10):4129-33. doi: 10.7314/apjcp.2014.15.10.4129.
The objective was to study the effect of Scutellaria baicalensis Georgi ethanol extracts (SBGE) on immune and anti-oxidant function in U14 tumor-bearing mice.
U14 tumor-bearing mice were randomly divided into eight groups: a control group, a cyclophosphamide (CTX) group, three dose groups of SBGEI (high, medium, low), and three dose groups of SBGEII (high, medium, low). After two weeks, the thymus and spleen weight indices of mice bearing U14 cervical cancer were calculated. Enzyme linked immunosorbent assays (ELISA) was used to determine the levels of serum IL-2, TNF-α, IL-8, and PCNA. MDA activity and SOD activity in plasma were measured with detection kits.
In the SBGE groups, thymus weight and spleen weight indices of U14 tumor-bearing mice were significantly higher than in the control group or CTX group (p<0.05). Compared to control group, the levels of serum IL-2 and TNF-α in U14 tumor-bearing mice increased significantly, whereas the contents of serum IL-8 and PCNA decreased (p<0.05). The activity of SOD increased with the growing dose of SBGE, while the activity of MDA decreased significantly in the higher- dose groups of SBGE.
These findings suggested that SBGE, especially at high dose, 1000 mg/kg, showed significant immune and anti-oxidant effects in U14 tumor-bearing mice, which might be the mechanisms of SBGE inhibition of tumor growth.
目的是研究黄芩乙醇提取物（SBGE）对U14荷瘤小鼠免疫和抗氧化功能的影响。
将U14荷瘤小鼠随机分为八组：对照组、环磷酰胺（CTX）组、三个剂量组的SBGEI（高、中、低）和三个剂量组的SBGEII（高、中、低）。两周后，计算U14宫颈癌荷瘤小鼠的胸腺和脾脏重量指数。采用酶联免疫吸附测定（ELISA）法测定血清白细胞介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）和增殖细胞核抗原（PCNA）水平。用检测试剂盒测定血浆中丙二醛（MDA）活性和超氧化物歧化酶（SOD）活性。
在SBGE组中，U14荷瘤小鼠的胸腺重量和脾脏重量指数显著高于对照组或CTX组（p<0.05）。与对照组相比，U14荷瘤小鼠血清IL-2和TNF-α水平显著升高，而血清IL-8和PCNA含量降低（p<0.05）。SOD活性随SBGE剂量增加而升高，而在较高剂量SBGE组中MDA活性显著降低。
这些结果表明，SBGE，尤其是1000mg/kg的高剂量，对U14荷瘤小鼠具有显著的免疫和抗氧化作用，这可能是SBGE抑制肿瘤生长的机制。
