Wang Chao, Ma Hong-Xi, Jin Mei-Shan, Zou Ya-Bin, Teng Yong-Liang, Tian Zhuang, Wang Hai-Ying, Wang Yin-Ping, Duan Xiu-Mei
Pathological Diagnosis Centre, The First Hospital Affiliated to Bethune Medical College, Jilin University, Changchun, Jilin, China E-mail :
Asian Pac J Cancer Prev. 2014;15(10):4187-92. doi: 10.7314/apjcp.2014.15.10.4187.
Matrix metalloproteinase (MMP)-2 and MMP-9 are important proteases involved in invasion and metastasis of various tumors. Extra-gastrointestinal stromal tumors (EGISTs) are rare neoplasms. This study was performed to assess MMP-2 and MMP-9 expression in EGIST tissue samples for association with clinicopathological data from the patients. Twenty-one surgical EGIST tissue specimens were collected for analysis of MMP-2 and MMP- 9 expression using immunohistochemistry. MMP-2 and MMP-9 proteins were expressed in all of the epithelial cell types of EGISTs, whereas they were only expressed in 75% of the spindle cell type, although there was no statistically significant difference (p>0.05). Expression of MMP-2 and MMP-9 proteins was associated with tumor size, mitotic rate, tumor necrosis, and distant metastasis (p<0.05). MMP-2 expression was linked with MMP-9 levels (p<0.05). However, there was no correlation between MMP-9 expression and age, sex, primary site, or cell morphology in any of these 21 EGIST patients (p>0.05). Moreover, expression of MMP-2 and MMP-9 proteins increased with the degree of EGIST risk. This study provided evidence of an association of MMP-2 and MMP-9 expression with advanced EGIST behavior.
基质金属蛋白酶（MMP）-2和MMP-9是参与各种肿瘤侵袭和转移的重要蛋白酶。胃肠道外间质瘤（EGIST）是罕见的肿瘤。本研究旨在评估EGIST组织样本中MMP-2和MMP-9的表达，并将其与患者的临床病理数据相关联。收集了21份手术切除的EGIST组织标本，采用免疫组织化学法分析MMP-2和MMP-9的表达。MMP-2和MMP-9蛋白在EGIST的所有上皮细胞类型中均有表达，而在梭形细胞类型中仅75%表达，尽管差异无统计学意义（p>0.05）。MMP-2和MMP-9蛋白的表达与肿瘤大小、有丝分裂率、肿瘤坏死和远处转移相关（p<0.05）。MMP-2的表达与MMP-9水平相关（p<0.05）。然而，在这21例EGIST患者中，MMP-9的表达与年龄、性别、原发部位或细胞形态均无相关性（p>0.05）。此外，MMP-2和MMP-9蛋白的表达随EGIST风险程度增加。本研究提供了MMP-2和MMP-9表达与晚期EGIST行为相关的证据。
