Fu Lin, Liu Nan, Han Yong, Xie Chengyao, Li Qingchang, Wang Enhua
Department of Pathology, College of Basic Medical Sciences, The First Affiliated Hospital and China Medical University, Shenyang, 110001, China.
Tumour Biol. 2014 Sep;35(9):9263-8. doi: 10.1007/s13277-014-2201-9. Epub 2014 Jun 18.
A disintegrin and metalloprotease 10 (ADAM10) is upregulated in several cancers and associates with malignant cancer progression. However, its expression pattern in bladder cancer remains unexplored. In the present study, we examined ADAM10 expression in 105 bladder cancer specimens using immunohistochemistry. We found ADAM10 overexpression in 51 of 105 (48.5 %) bladder cancer specimens. ADAM10 overexpression associated with advanced tumor stage (p = 0.001) and tumor grade (p = 0.018). To explore its biological functions in bladder cancer cells, small interfering RNA (siRNA) knockdown was performed in 5,637 and T24 cell lines. Cell Counting Kit-8 (CCK8) assay and Matrigel invasion assay showed that ADAM10 depletion decreased cell proliferation, migration, and invasion. In addition, ADAM10 knockdown increased the level of cisplatin-induced apoptosis. In conclusion, ADAM10 is overexpressed in bladder cancer and regulates malignant cell growth and invasion, which makes it a candidate therapeutic target.
解整合素金属蛋白酶10（ADAM10）在多种癌症中表达上调，并与恶性肿瘤进展相关。然而，其在膀胱癌中的表达模式仍未被探索。在本研究中，我们使用免疫组织化学方法检测了105例膀胱癌标本中ADAM10的表达。我们发现105例膀胱癌标本中有51例（48.5%）ADAM10过表达。ADAM10过表达与肿瘤晚期（p = 0.001）和肿瘤分级（p = 0.018）相关。为了探究其在膀胱癌细胞中的生物学功能，我们在5637和T24细胞系中进行了小干扰RNA（siRNA）敲低实验。细胞计数试剂盒-8（CCK8）检测和基质胶侵袭实验表明，ADAM10缺失会降低细胞增殖、迁移和侵袭能力。此外，ADAM10敲低会增加顺铂诱导的细胞凋亡水平。总之，ADAM10在膀胱癌中过表达，并调节恶性细胞的生长和侵袭，这使其成为一个潜在的治疗靶点。
