Institut de Recherche pour le Développement (IRD), UMR226 ISE-M, Bât. 22 - CC065, Place Eugène Bataillon, 34095, Montpellier Cedex 5, France.
Mol Ecol Resour. 2015 Jan;15(1):57-67. doi: 10.1111/1755-0998.12293. Epub 2014 Jul 18.
Marine fishes exhibit spectacular phenotypic changes during their ontogeny, and the identification of their early stages is challenging due to the paucity of diagnostic morphological characters at the species level. Meanwhile, the importance of early life stages in dispersal and connectivity has recently experienced an increasing interest in conservation programmes for coral reef fishes. This study aims at assessing the effectiveness of DNA barcoding for the automated identification of coral reef fish larvae through large-scale ecosystemic sampling. Fish larvae were mainly collected using bongo nets and light traps around Moorea between September 2008 and August 2010 in 10 sites distributed in open waters. Fish larvae ranged from 2 to 100 mm of total length, with the most abundant individuals being <5 mm. Among the 505 individuals DNA barcoded, 373 larvae (i.e. 75%) were identified to the species level. A total of 106 species were detected, among which 11 corresponded to pelagic and bathypelagic species, while 95 corresponded to species observed at the adult stage on neighbouring reefs. This study highlights the benefits and pitfalls of using standardized molecular systems for species identification and illustrates the new possibilities enabled by DNA barcoding for future work on coral reef fish larval ecology.
海洋鱼类在其个体发育过程中表现出惊人的表型变化，由于在物种水平上缺乏诊断形态特征，因此鉴定其早期阶段具有挑战性。与此同时，早期生活阶段在珊瑚礁鱼类的扩散和连通性中的重要性最近在保护计划中受到越来越多的关注。本研究旨在评估 DNA 条形码在通过大规模生态系统采样自动识别珊瑚礁鱼幼虫方面的有效性。2008 年 9 月至 2010 年 8 月，在莫雷阿岛的 10 个地点使用邦戈网和灯光陷阱在开阔水域中采集了主要的鱼类幼虫。鱼类幼虫全长 2 至 100 毫米，最丰富的个体<5 毫米。在 505 个 DNA 条形码个体中，有 373 个幼虫（即 75%）被鉴定到种的水平。共检测到 106 种，其中 11 种为浮游和深海物种，95 种为在邻近珊瑚礁上观察到的成鱼。本研究强调了使用标准化分子系统进行物种鉴定的好处和陷阱，并说明了 DNA 条形码为未来珊瑚礁鱼类幼虫生态学工作带来的新可能性。
