Habib Kazi Ahsan, Islam Md Jayedul, Sakib Md Nazmus, Brishti Parsha Shanjana, Neogi Amit Kumer
Department of Fisheries Biology and Genetics, Faculty of Fisheries, Aquaculture and Marine Science Sher-e-Bangla Agricultural University Dhaka Bangladesh.
Aquatic Bioresource Research Lab, Department of Fisheries Biology and Genetics Sher-e-Bangla Agricultural University Dhaka Bangladesh.
Ecol Evol. 2023 Oct 22;13(10):e10641. doi: 10.1002/ece3.10641. eCollection 2023 Oct.
This study employs the DNA barcoding approach to make a molecular taxonomic catalog of reef fishes of Saint Martin's Island (SMI), an ecologically critical area (ECA), and Marine Protected Area (MPA) in Bangladesh. DNA barcoding, along with morphological analysis, confirmed 84 reef-associated fish species in SMI belonging to 16 orders, 39 families, and 67 genera. A total of 184 sequences were obtained in this study where 151 sequences (534-604 bp) of 81 species were identified from the COI barcode gene and 33 sequences (609 bp) of 19 species from the 16S rRNA gene region which were submitted to the GenBank and Barcode of Life Data System (BOLD). Among these sequences, 70 sequences of the COI gene and 16 sequences of 16S rRNA gene region from 41 species were submitted for the first time into the GenBank from Bangladesh. For molecular characterization analysis, another 37 sequences of 15 reef fish species of SMI were added from previous studies, making a total of 221 DNA sequences which comprised 179 sequences of 96 species for the COI gene and 42 sequences of 26 species for the 16S rRNA gene region. The COI sequences contain 145 haplotypes with 337 polymorphic sites, and the mean genetic distances within species, genera, and families were calculated as 0.34%, 12.26%, and 19.03%, respectively. On the contrary, 16S rRNA sequences comprised 31 haplotypes with 241 polymorphic sites, and the mean genetic divergences within species, genera, and families were 0.94%, 4.72%, and 12.43%, respectively. This study is a significant contribution to the marine biodiversity of Bangladesh which would facilitate the assessment of species diversity for strategizing management action. It is also an important input to the DNA barcode library of reef fishes of the northern Bay of Bengal.
本研究采用DNA条形码方法，对孟加拉国生态关键区域（ECA）和海洋保护区（MPA）圣马丁岛（SMI）的珊瑚礁鱼类进行分子分类编目。DNA条形码结合形态学分析，确认了SMI中84种与珊瑚礁相关的鱼类，它们隶属于16目、39科和67属。本研究共获得184条序列，其中从细胞色素氧化酶亚基I（COI）条形码基因中鉴定出81个物种的151条序列（534 - 604 bp），从16S核糖体RNA（rRNA）基因区域鉴定出19个物种的33条序列（609 bp），这些序列已提交至GenBank和生命条形码数据系统（BOLD）。在这些序列中，来自41个物种的70条COI基因序列和16S rRNA基因区域的16条序列首次从孟加拉国提交至GenBank。为进行分子特征分析，从先前的研究中又添加了SMI的15种珊瑚礁鱼类的37条序列，总共得到221条DNA序列，其中包括96个物种的179条COI基因序列和26个物种的42条16S rRNA基因区域序列。COI序列包含145个单倍型，有337个多态性位点，种内、属内和科内的平均遗传距离分别计算为0.34%、12.26%和19.03%。相反，16S rRNA序列包含31个单倍型，有241个多态性位点，种内、属内和科内的平均遗传差异分别为0.94%、4.72%和12.43%。本研究对孟加拉国的海洋生物多样性做出了重大贡献，这将有助于评估物种多样性，为制定管理行动策略提供依据。它也是孟加拉湾北部珊瑚礁鱼类DNA条形码文库的重要补充。
