Lima Moema Cristina Costa de, Lima Same Costa, Savada Camila Satie, Suzuki Karen Mayumi, Orsi Mário Luís, Almeida Fernanda Simões de
Universidade Estadual de Londrina, Departamento de Biologia Geral, Laboratório de Genética e Ecologia Animal, Londrina, PR, Brazil.
Universidade Estadual de Londrina, Departamento de Biologia Animal e Vegetal, Laboratório de Ecologia de Peixes e Invasões Biológicas, Londrina, PR, Brazil.
Genet Mol Biol. 2020 Jun 22;43(3):e20190352. doi: 10.1590/1678-4685-GMB-2019-0352. eCollection 2020.
Fish eggs are often excluded from identification analysis since at this stage of development there are few morphological characters. The correct identification of eggs can provide important information about spawning areas of species. The current work aimed to identify fish eggs in the Tibagi and Cinzas Rivers using the DNA barcode to obtain information on richness and diversity, adding to the existing data in the area. Of the 928 sequences analyzed using the BOLD Systems database, 99.78% were able to be identified at a specific level, demonstrating a high success rate for egg identification. The samples resulted in 25 species, 11 families, and 2 orders. Of the 25 species found, more than half (60%) present reproductive migration behavior, indicating that the tributaries of the Capivara reservoir are being used as a migratory route by these species. Eggs of rare and endangered species were found, indicating these tributaries as spawning grounds for these species. The results demonstrate the importance of identifying fish eggs in reservoir-influenced environments to recognize breeding areas of native and endangered species, as well as the importance of the Tibagi and Cinzas Rivers for the maintenance of native fish species in the Paranapanema River.
鱼卵通常被排除在鉴定分析之外，因为在这个发育阶段几乎没有形态特征。正确鉴定鱼卵可以提供有关物种产卵区域的重要信息。当前的工作旨在利用DNA条形码鉴定蒂巴吉河和辛扎斯河中的鱼卵，以获取有关丰富度和多样性的信息，补充该地区的现有数据。在使用BOLD系统数据库分析的928个序列中，99.78%能够在特定水平上被鉴定出来，表明鱼卵鉴定的成功率很高。样本共鉴定出25个物种、11个科和2个目。在发现的25个物种中，超过一半（60%）呈现繁殖洄游行为，这表明卡皮瓦拉水库的支流正被这些物种用作洄游路线。发现了珍稀和濒危物种的鱼卵，表明这些支流是这些物种的产卵地。结果表明，在受水库影响的环境中鉴定鱼卵对于识别本地和濒危物种的繁殖区域非常重要，同时也表明蒂巴吉河和辛扎斯河对于巴拉那帕内马河本地鱼类物种的维持具有重要意义。
