钙干预可改善多发性硬化症的实验模型。
Calcium intervention ameliorates experimental model of multiple sclerosis.
作者信息
Haghmorad Dariush, Mahmoudi Mohammad B, Mahmoudi Mahmoud, Rab Shahrzad Z T, Rastin Maryam, Shegarfi Hamid, Azizi Gholamreza, Mirshafiey Abbas
机构信息
Immunology Research Center, Buali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Genetics department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
Oman Med J. 2014 May;29(3):185-9. doi: 10.5001/omj.2014.46.
OBJECTIVE
Multiple sclerosis (MS) is the most common inflammatory disease of the CNS. Experimental autoimmune encephalomyelitis (EAE) is a widely used model for MS. In the present research, our aim was to test the therapeutic efficacy of Calcium (Ca) in an experimental model of MS.
METHODS
In this study the experiment was done on C57BL/6 mice. EAE was induced using 200 μg of the MOG35-55 peptide emulsified in CFA and injected subcutaneously on day 0 over two flank areas. In addition, 250 ng of pertussis toxin was injected on days 0 and 2. In the treatment group, 30 mg/kg Ca was administered intraperitoneally four times at regular 48 hour intervals. The mice were sacrificed 21 days after EAE induction and blood samples were taken from their hearts. The brains of mice were removed for histological analysis and their isolated splenocytes were cultured.
RESULTS
Our results showed that treatment with Ca caused a significant reduction in the severity of the EAE. Histological analysis indicated that there was no plaque in brain sections of Ca treated group of mice whereas 4 ± 1 plaques were detected in brain sections of controls. The density of mononuclear infiltration in the CNS of Ca treated mice was lower than in controls. The serum level of Nitric Oxide in the treatment group was lower than in the control group but was not significant. Moreover, the levels of IFN-γ in cell culture supernatant of splenocytes in treated mice were significantly lower than in the control group.
CONCLUSION
The data indicates that Ca intervention can effectively attenuate EAE progression.
目的
多发性硬化症（MS）是中枢神经系统最常见的炎症性疾病。实验性自身免疫性脑脊髓炎（EAE）是一种广泛应用于MS的模型。在本研究中，我们的目的是在MS实验模型中测试钙（Ca）的治疗效果。
方法
在本研究中，实验在C57BL/6小鼠上进行。使用乳化于完全弗氏佐剂（CFA）中的200μg髓鞘少突胶质细胞糖蛋白35-55（MOG35-55）肽在第0天皮下注射于两个胁腹区域诱导EAE。此外，在第0天和第2天注射250ng百日咳毒素。在治疗组中，以48小时间隔腹腔注射30mg/kg Ca共4次。在EAE诱导后21天处死小鼠，并从其心脏采集血样。取出小鼠的脑进行组织学分析，并培养分离的脾细胞。
结果
我们的结果表明，用Ca治疗可显著降低EAE的严重程度。组织学分析表明，Ca治疗组小鼠的脑切片中无斑块，而对照组脑切片中检测到4±1个斑块。Ca治疗小鼠中枢神经系统中的单核细胞浸润密度低于对照组。治疗组血清一氧化氮水平低于对照组，但差异不显著。此外，治疗组小鼠脾细胞培养上清液中干扰素-γ水平显著低于对照组。
结论
数据表明Ca干预可有效减轻EAE进展。
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